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AB313689

Human NPP-7 ELISA Kit

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Human NPP-7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NPP-7 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ3077/PRO9912, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase

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Sandwich ELISA - Human NPP-7 ELISA Kit (AB313689)
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Supplier Data

Sandwich ELISA - Human NPP-7 ELISA Kit (AB313689)

Example of standard curve using ab313689. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22.5 pg/mL

Range

22.5 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NPP-7 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NPP-7 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human NPP-7 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A,
IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO-1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP2, TSLP R, UNC5H4.

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Recovery

[ { "sample": "Serum", "range": "84 - 93 %", "average": "= 88.38" }, { "sample": "Plasma", "range": "79 - 92 %", "average": "= 86.05" }, { "sample": "Cell culture media", "range": "76 - 83 %", "average": "= 80.68" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313689-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human NPP-7 Protein Standard", "number":"AB313689-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313689-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human NPP-7 Detection Antibody", "number":"AB313689-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313689-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313689-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313689-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313689-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human NPP-7 Antibody-coated ELISA Plate", "number":"AB313689-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPP-7 also known as nucleotide pyrophosphatase/phosphodiesterase 7 is an enzyme involved in the hydrolysis of pyrophosphate-containing substrates. It has a molecular mass of approximately 125 kDa. This enzyme exhibits expression in several human tissues prominently in the intestinal tract and kidney. The enzyme's activity facilitates the breakdown of molecules serving roles in both digestion and cellular signaling processes.
Biological function summary

NPP-7 participates in lipid metabolism and contributes to maintaining homeostasis by degrading extracellular nucleotides. It forms part of larger protein complexes that regulate various cellular activities. Its enzymatic function is important for controlling the concentration of lipid-derived signaling molecules thereby impacting cellular communication and response mechanisms.

Pathways

NPP-7 integrates into the pathway regulating phospholipid metabolism and nucleotide salvage pathways. It interacts with proteins such as NPP-2 which are involved in similar metabolic processes ensuring balance in lipid signaling and energy storage within cells. Its function in these pathways highlights its role in modulating phospholipid levels and influencing liver function and cellular energy dynamics.

NPP-7 relates to conditions such as metabolic syndrome and chronic kidney disease. Dysregulation or altered expression of NPP-7 can affect lipid metabolism and kidney function contributing to these diseases. This protein interacts with other proteins including ENPP1 which ties into its involvement in disrupting normal cellular metabolism and exacerbating pathological states in these conditions.
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Product protocols

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Target data

Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract (PubMed : 12671034, PubMed : 12885774, PubMed : 15205117, PubMed : 16255717, PubMed : 28292932). Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation (PubMed : 12885774, PubMed : 16255717). Does not have nucleotide pyrophosphatase activity (PubMed : 12885774). May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen (By similarity).
See full target information ENPP7
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websiteProtocolBooklet
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