Human NPP-7 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human NPP-7 in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 22.5 - 6000 pg/mL
- Sensitivity
- = 22.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract (PubMed:12671034, PubMed:12885774, PubMed:15205117, PubMed:16255717, PubMed:28292932). Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation (PubMed:12885774, PubMed:16255717). Does not have nucleotide pyrophosphatase activity (PubMed:12885774). May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen (By similarity).
Alternative names
UNQ3077/PRO9912, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase
What's included?
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Human NPP-7 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human NPP-7 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 22.5 - 6000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 22.5 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88.38 | Range 84 - 93 % |
Sample type Plasma | Average % = 86.05 | Range 79 - 92 % |
Sample type Cell culture media | Average % = 80.68 | Range 76 - 83 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human NPP-7 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NPP-7 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human NPP-7 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A,
IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO-1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP2, TSLP R, UNC5H4.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NPP-7 also known as nucleotide pyrophosphatase/phosphodiesterase 7 is an enzyme involved in the hydrolysis of pyrophosphate-containing substrates. It has a molecular mass of approximately 125 kDa. This enzyme exhibits expression in several human tissues prominently in the intestinal tract and kidney. The enzyme's activity facilitates the breakdown of molecules serving roles in both digestion and cellular signaling processes.
- Biological function summary
NPP-7 participates in lipid metabolism and contributes to maintaining homeostasis by degrading extracellular nucleotides. It forms part of larger protein complexes that regulate various cellular activities. Its enzymatic function is important for controlling the concentration of lipid-derived signaling molecules thereby impacting cellular communication and response mechanisms.
- Pathways
NPP-7 integrates into the pathway regulating phospholipid metabolism and nucleotide salvage pathways. It interacts with proteins such as NPP-2 which are involved in similar metabolic processes ensuring balance in lipid signaling and energy storage within cells. Its function in these pathways highlights its role in modulating phospholipid levels and influencing liver function and cellular energy dynamics.
- Associated diseases and disorders
NPP-7 relates to conditions such as metabolic syndrome and chronic kidney disease. Dysregulation or altered expression of NPP-7 can affect lipid metabolism and kidney function contributing to these diseases. This protein interacts with other proteins including ENPP1 which ties into its involvement in disrupting normal cellular metabolism and exacerbating pathological states in these conditions.
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1 product image
Sandwich ELISA - Human NPP-7 ELISA Kit (ab313689)
Example of standard curve using ab313689. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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