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AB314847

Human NPTX2 ELISA Kit - Intracellular

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Human NPTX2 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human NPTX2 with a sensitivity of 12.535 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Neuronal pentraxin-2, NP2, Neuronal pentraxin II, NP-II, NPTX2

3 Images
Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)
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Supplier Data

Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)

Example of human NPTX2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)
  • sELISA

Supplier Data

Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)

Interpolated concentration of native NPTX2 was measured in duplicate. Undiluted samples are 100 µg/mL human cortex tissue extract, mouse whole brain tissue extract, and rat whole brain tissue extract.  The interpolated values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR.

Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)
  • sELISA

Supplier Data

Sandwich ELISA - Human NPTX2 ELISA Kit - Intracellular (AB314847)

Interpolated concentration of native NPTX2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : SH-SY5Y cell extract 200 µg/mL and human cortex tissue extract 125 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.535 pg/mL

Range

40.625 - 2600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB277533

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BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (AB277533)

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Biochemicals

AB146592

PD 98059 (DMSO solution), MEK1 inhibitor

Chemical Structure - PD 98059 (DMSO solution), MEK1 inhibitor (AB146592)

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Primary Antibodies

AB272574

Anti-SQRDL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQRDL antibody (AB272574)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NPTX2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NPTX2 protein in Cell Lysate, Tissue extracts. Quantitate Human NPTX2 with 12.535 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.09" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "81 - 106 %", "average": "= 95" }, { "sample": "Tissue Extracts", "range": "90 - 107 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314847-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314847-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314847-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314847-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314847-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NPTX2 Lyophilized Recombinant Protein", "number":"AB314847-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB314847-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NPTX2 Capture Antibody", "number":"AB314847-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NPTX2 Detector Antibody", "number":"AB314847-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB314847-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314847-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314847-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPTX2 protein also known as neuronal pentraxin II or NARP weighs approximately 47 kDa. NPTX2 is expressed mostly in the brain especially in neurons. This protein belongs to the neuronal pentraxin family and plays a mechanical role in processes like synaptic plasticity. NPTX2 operates by binding to AMPA receptors influencing their surface expression.
Biological function summary

NPTX2 affects synaptic stabilization by participating in the formation and maintenance of synaptic connections. It forms complexes with AMPA receptor subunits promoting synapses that are more active and facilitating synaptic communication. NPTX2's involvement is essential for normal synaptic function reflecting its significance in neural signaling.

Pathways

NPTX2 plays a role in the glutamatergic signaling pathway an important pathway for synaptic transmission involving excitatory neurotransmitters. NPTX2 works alongside other proteins like GluR1 subunit in this pathway mediating synaptic responses. Through its actions in these pathways NPTX2 contributes to long-term potentiation a process related to learning and memory.

NPTX2 has links to neurological conditions such as Alzheimer’s disease and schizophrenia. In Alzheimer's NPTX2 dysregulation associates with synaptic dysfunction possibly involving interactions with beta-amyloid. In schizophrenia altered NPTX2 expression might contribute to glutamatergic signaling imbalances affecting cognitive function. These associations highlight NPTX2's importance in maintaining normal neural processes.
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Product protocols

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Target data

Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).
See full target information NPTX2
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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