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AB277467

Human NPTXR ELISA Kit

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Human NPTXR ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NPTXR in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Neuronal pentraxin receptor, NPTXR

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ELISA - Human NPTXR ELISA Kit (AB277467)
  • ELISA

Supplier Data

ELISA - Human NPTXR ELISA Kit (AB277467)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.4 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NPTXR ELISA Kit (ab277467) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NPTXR in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human NPTXR coated on a 96-well plate. Standards and samples are pipetted into the wells and Human NPTXR present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human NPTXR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human NPTXR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 78 %", "average": "= 73.88" }, { "sample": "Plasma", "range": "68 - 90 %", "average": "= 75.32" }, { "sample": "Cell culture media", "range": "68 - 94 %", "average": "= 78.46" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277467-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277467-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277467-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human NPTXR coated Microplate", "number":"AB277467-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human NPTXR Antibody", "number":"AB277467-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB277467-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277467-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277467-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NPTXR standard protein (Lyophilized)", "number":"AB277467-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277467-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPTXR also known as Neuronal Pentraxin Receptor is a protein involved in the synaptic organization. It has a molecular mass of approximately 65 kDa. NPTXR expresses mainly in the central nervous system including the brain and spinal cord. As a synaptic protein it plays a role in modulating neuron-neuron interactions and signaling pathways critical for the central nervous system's proper function.
Biological function summary

Neuronal pentraxin receptor contributes to synaptic plasticity and maintenance. It forms a complex with other pentraxin proteins like NPTX1 and NPTX2 facilitating the clustering of AMPA receptors at synapses. This interaction enhances excitatory synaptic transmission which is important for processes like learning and memory. NPTXR therefore plays a significant role in maintaining synaptic strength and integrity.

Pathways

The neuronal pentraxin receptor influences the glutamatergic signaling pathway. This pathway involves neurotransmitter glutamate and its receptors which include AMPA-type glutamate receptors at postsynaptic sites. NPTXR's interaction with AMPA receptors via NPTX1 and NPTX2 promotes receptor clustering critical for NMDA receptor phosphorylation. As a result this protein contributes to synaptic plasticity influencing learning and memory pathways in neurons.

NPTXR has implications in neurodegenerative diseases and schizophrenia. Altered synaptic plasticity and receptor clustering can lead to cognitive deficits frequently observed in Alzheimer's disease. Additionally studies suggest a potential link between NPTXR and schizophrenia where dysregulated synaptic organization impacts neurotransmission. NPTXR's association with AMPA receptors through other pentraxins implicates it in these disorders highlighting its significance as a therapeutic target.
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Product protocols

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Target data

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
See full target information NPTXR
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websiteProtocolBooklet
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