JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267568

Human NrCAM ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human NrCAM ELISA Kit is a Sandwich ELISA for the measurement of Human NrCAM in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

KIAA0343, NRCAM, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, hBravo, Ng-CAM-related

1 Images
Sandwich ELISA - Human NrCAM ELISA Kit (AB267568)
  • sELISA

Supplier Data

Sandwich ELISA - Human NrCAM ELISA Kit (AB267568)

Human NrCAM ELISA kit (ab267568) Standard curves in Assay Diluent A and Assay Diluent D.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.3 ng/mL

Range

0.328 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB191418

Anti-NrCAM antibody [EPR15654] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

  • 0
  • 0
Multiplex Kits

AB169815

Human Cytokine Antibody Array - Membrane (51 targets)

Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (51 targets) (AB169815)

  • 0
  • 0
Multiplex Kits

AB197434

Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative

Multiplex Protein Detection - Human Adhesion Molecule Antibody Array (17 Targets) - Quantitative (AB197434)

  • 0
  • 0
Multiplex Kits

AB197428

Human Cytokine Antibody Array (200 Targets) - Quantitative

Multiplex Protein Detection - Human Cytokine Antibody Array (200 Targets) - Quantitative (AB197428)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human NrCAM ELISA Kit is designed for the quantitative determination of NRCAM in cell culture supernatants, plasma and serum samples.

ΔNote: Human NrCAM concentration is low in normal serum and plasma samples and may not be detectable in this assay.

This assay employs an antibody specific for human NrCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and NrCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human NrCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NrCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "68 - 143 %", "average": "= 108" }, { "sample": "Plasma", "range": "68 - 106 %", "average": "= 79" }, { "sample": "Cell culture media", "range": "99 - 133 %", "average": "= 115" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267568-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267568-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267568-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human NrCAM coated Microplate (12 x 8 wells)", "number":"AB267568-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267568-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267568-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267568-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB267568-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human NrCAM Detection Antibody", "number":"AB267568-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267568-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NrCAM Standard (Lyophilized)", "number":"AB267568-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.
Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.
style
left-column

Product protocols

style
left-column

Target data

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.
See full target information NRCAM
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com