Human NRG1 beta 1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human NRG1 beta 1 in Human in Plasma, Cell culture supernatant samples.
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- Endocrine and metabolic science 3:2021Elevated neuregulin-1 levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Chambliss,Tatayana Richardson,John Onyekaba,Juan Cespedes,Annette Nti,Keri Oxendine Harp,Iris Buchanan-Perry,Jonathan K Stiles,Beatrice E GeePubMed 35935682
- Aging 12:5516-55382020Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarhana Ferdousi et. al.PubMed 32224504
- The International journal of biological markers 32:e333-e3362017Circulating neuregulin-1 and galectin-3 can be prognostic markers in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca De Iuliis,Gerardo Salerno,Ludovica Taglieri,Rosina Lanza,Patrizia Cardelli,Susanna ScarpaPubMed 28430337
- PloS one 11:e01487112016Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabine Stindt et. al.PubMed 26886748
- The Journal of investigative dermatology 135:3115-31242015BRAF Inhibition Generates a Host-Tumor Niche that Mediates Therapeutic Escape.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInna V Fedorenko et. al.PubMed 26302068
- Circulation. Heart failure 7:619-262014A bioengineered hydrogel system enables targeted and sustained intramyocardial delivery of neuregulin, activating the cardiomyocyte cell cycle and enhancing ventricular function in a murine model of ischemic cardiomyopathy.Applications:sELISAReactive species:Unspecified reactive speciesJeffrey E Cohen et. al.PubMed 24902740
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 82.3 - 20000 pg/mL
- Sensitivity
- < 50 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Human NRG1 beta 1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human NRG1 beta 1 in Human in Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 82.3 - 20000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 50 pg/mL
- General recovery
- 99%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 114.9 | Range 102 - 123 % |
Sample type Plasma | Average % = 86.02 | Range 79 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.
The ab100614 kit recognises the human HRG beta 1 protein (isoform 6) and has not been tested with other isoforms.
Optimization may be required with urine samples
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NRG1 beta 1 protein also known as neuregulin 1 or NRG1 plays an essential mechanical role in cellular communication and growth. It is part of a family of proteins involved in cell signaling and it exists in several isoforms one being NRG1 beta 1. The mass of NRG1 beta 1 is variable due to its different isoforms typically ranging from 44 to 47 kDa. This protein is expressed in various tissues including the heart brain and other neural tissues indicating its importance in both peripheral and central systems.
- Biological function summary
NRG1 beta 1 significantly influences the development and function of the nervous system. It interacts with the ErbB family of receptors to form a complex that plays a role in cellular proliferation and differentiation. This interaction is important for synaptic plasticity and the formation of neuromuscular junctions. NRG1 beta 1 also contributes to the maintenance of heart function by regulating growth and repair processes in cardiac muscle cells.
- Pathways
Researchers recognize NRG1 beta 1 as an important component in the PI3K/AKT and MAPK signaling pathways. These pathways are important for regulating cell survival growth and differentiation. Through its binding with ErbB receptors NRG1 beta 1 activates these pathways influencing downstream effectors. Research has shown a relationship between NRG1 and other signaling proteins such as neurotrophins indicating its central role in neurodevelopmental processes.
- Associated diseases and disorders
NRG1 beta 1 links to conditions like schizophrenia and heart failure. Altered NRG1 signaling has been associated with schizophrenia where it interacts with ErbB4 receptors showing changes in cognitive function and synaptic connectivity. In cardiac diseases including heart failure dysregulation of NRG1 beta 1 affects cardiac muscle cells' repair interacting with proteins like ErbB2. These relationships highlight the importance of NRG1 beta 1 in both neural and cardiac health contexts.
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ELISA - Human NRG1 beta 1 ELISA Kit (ab100614)
NRG1 beta measured in human biological fluids; signals from serum and from rat and mouse samples were below level of detection (duplicates +/- SD).
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