AB100614

Human NRG1 beta 1 ELISA Kit

Name: Human NRG1 beta 1 ELISA Kit (ab100614) | Abcam
Brand: Abcam Limited
SKU: AB100614
Price: 780 USD
Availability: InStock

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(6 Publications)

Human NRG1 beta 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NRG1 beta 1 in Human in Biofluids, Cell Culture Media samples.

1 Images

ELISA - Human NRG1 beta 1 ELISA Kit (AB100614)

ELISA

Lab

ELISA - Human NRG1 beta 1 ELISA Kit (AB100614)

NRG1 beta measured in human biological fluids; signals from serum and from rat and mouse samples were below level of detection (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 50 pg/mL

Range

82.3 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.

The ab100614 kit recognises the human HRG beta 1 protein (isoform 6) and has not been tested with other isoforms.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 123 %", "average": "= 114.9" }, { "sample": "Plasma", "range": "79 - 94 %", "average": "= 86.02" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100614-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100614-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100614-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"NRG1 beta 1 Microplate (12 x 8 wells)", "number":"AB100614-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100614-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human NRG1 beta 1", "number":"AB100614-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100614-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human NRG1 beta 1 Standard (lyophilized)", "number":"AB100614-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100614-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100614-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NRG1 beta 1 protein also known as neuregulin 1 or NRG1 plays an essential mechanical role in cellular communication and growth. It is part of a family of proteins involved in cell signaling and it exists in several isoforms one being NRG1 beta 1. The mass of NRG1 beta 1 is variable due to its different isoforms typically ranging from 44 to 47 kDa. This protein is expressed in various tissues including the heart brain and other neural tissues indicating its importance in both peripheral and central systems.

Biological function summary

NRG1 beta 1 significantly influences the development and function of the nervous system. It interacts with the ErbB family of receptors to form a complex that plays a role in cellular proliferation and differentiation. This interaction is important for synaptic plasticity and the formation of neuromuscular junctions. NRG1 beta 1 also contributes to the maintenance of heart function by regulating growth and repair processes in cardiac muscle cells.

Pathways

Researchers recognize NRG1 beta 1 as an important component in the PI3K/AKT and MAPK signaling pathways. These pathways are important for regulating cell survival growth and differentiation. Through its binding with ErbB receptors NRG1 beta 1 activates these pathways influencing downstream effectors. Research has shown a relationship between NRG1 and other signaling proteins such as neurotrophins indicating its central role in neurodevelopmental processes.

NRG1 beta 1 links to conditions like schizophrenia and heart failure. Altered NRG1 signaling has been associated with schizophrenia where it interacts with ErbB4 receptors showing changes in cognitive function and synaptic connectivity. In cardiac diseases including heart failure dysregulation of NRG1 beta 1 affects cardiac muscle cells' repair interacting with proteins like ErbB2. These relationships highlight the importance of NRG1 beta 1 in both neural and cardiac health contexts.

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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Endocrine and metabolic science 3: PubMed35935682

2021

Elevated neuregulin-1 levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Chambliss,Tatayana Richardson,John Onyekaba,Juan Cespedes,Annette Nti,Keri Oxendine Harp,Iris Buchanan-Perry,Jonathan K Stiles,Beatrice E Gee

Aging 12:5516-5538 PubMed32224504

2020

Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Farhana Ferdousi,Shinji Kondo,Kazunori Sasaki,Yoshiaki Uchida,Nobuhiro Ohkohchi,Yun-Wen Zheng,Hiroko Isoda

The International journal of biological markers 32:e333-e336 PubMed28430337

2017

Circulating neuregulin-1 and galectin-3 can be prognostic markers in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca De Iuliis,Gerardo Salerno,Ludovica Taglieri,Rosina Lanza,Patrizia Cardelli,Susanna Scarpa

PloS one 11:e0148711 PubMed26886748

2016

Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Stindt,Patricia Cebula,Ute Albrecht,Verena Keitel,Jan Schulte am Esch,Wolfram T Knoefel,Ralf Bartenschlager,Dieter Häussinger,Johannes G Bode

The Journal of investigative dermatology 135:3115-3124 PubMed26302068

2015

BRAF Inhibition Generates a Host-Tumor Niche that Mediates Therapeutic Escape.

Applications

Unspecified application

Species

Unspecified reactive species

Inna V Fedorenko,Jennifer A Wargo,Keith T Flaherty,Jane L Messina,Keiran S M Smalley

Circulation. Heart failure 7:619-26 PubMed24902740

2014

A bioengineered hydrogel system enables targeted and sustained intramyocardial delivery of neuregulin, activating the cardiomyocyte cell cycle and enhancing ventricular function in a murine model of ischemic cardiomyopathy.

Applications

sELISA

Species

Unspecified reactive species

Jeffrey E Cohen,Brendan P Purcell,John W MacArthur,Anbin Mu,Yasuhiro Shudo,Jay B Patel,Christopher M Brusalis,Alen Trubelja,Alexander S Fairman,Bryan B Edwards,Mollie S Davis,George Hung,William Hiesinger,Pavan Atluri,Kenneth B Margulies,Jason A Burdick,Y Joseph Woo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human NRG1 beta 1 ELISA Kit

ELISA - Human NRG1 beta 1 ELISA Kit (AB100614)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Assay Kits

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Secondary Antibodies

Product promise