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AB277470

Human NRP2 ELISA kit

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Human NRP2 ELISA kit is a Sandwich ELISA for the measurement of Human NRP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

VEGF165R2, NRP2, Neuropilin-2, Vascular endothelial cell growth factor 165 receptor 2

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ELISA - Human NRP2 ELISA kit (AB277470)
  • ELISA

Supplier Data

ELISA - Human NRP2 ELISA kit (AB277470)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.1 ng/mL

Range

8.192 - 2000 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NRP2 ELISA Kit (ab277470) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human NRP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human NRP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human NRP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human NRP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "79 - 103 %", "average": "= 95.19" }, { "sample": "Plasma", "range": "68 - 106 %", "average": "= 81.76" }, { "sample": "Cell culture media", "range": "90 - 108 %", "average": "= 98.15" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277470-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277470-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277470-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277470-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human NRP2 coated Microplate", "number":"AB277470-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB277470-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human NRP2 Antibody", "number":"AB277470-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277470-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NRP2 standard protein (Lyophilized)", "number":"AB277470-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRP2 also known as Neuropilin-2 is a receptor protein involved in developmental processes including axon guidance and angiogenesis. The molecular mass of NRP2 is approximately 120 kDa. It is expressed in various tissues such as the nervous system and the vascular system but its expression can also be found in certain immune cells. NRP2 plays an important role in cellular signaling by binding to semaphorins and vascular endothelial growth factors (VEGF).
Biological function summary

NRP2 influences several processes including axonal guidance and the formation of blood vessels. It acts as a co-receptor by forming complexes with other proteins to mediate cellular responses. This role in signaling helps guide neuron growth and regulates vascular development. The NRP2-associated complexes often modulate responses to external signals contributing to the precise control of network formation in tissues.

Pathways

NRP2 plays a significant role in the VEGF signaling pathway and the semaphorin signaling pathway. These pathways are essential for cell growth migration and differentiation. NRP2 works closely with VEGF receptors like VEGFR2 to promote angiogenic responses. It also interacts with semaphorin family proteins to guide neuronal development. Together these pathways ensure the proper formation and function of neural and vascular networks.

NRP2 is connected to cancer progression and cardiovascular diseases. Altered expression of NRP2 has been observed in various cancer types where it may contribute to tumor growth and metastasis. In these diseases NRP2's interaction with its partners such as VEGFR2 becomes dysregulated affecting normal cellular processes. Cardiovascular conditions can also arise from issues in NRP2-related signaling where the normal function of blood vessel formation is compromised.
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Product protocols

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Target data

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.. (Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells.
See full target information NRP2
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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