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AB315040

Human NRP2 ELISA Kit - Extracellular

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Human NRP2 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human NRP2 with a sensitivity of 18.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

VEGF165R2, NRP2, Neuropilin-2, Vascular endothelial cell growth factor 165 receptor 2

4 Images
Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)
  • sELISA

Supplier Data

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)

Interpolated concentration of native NRP2 was measured in duplicate at different sample concentrations. Undiluted samples are 8% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)
  • sELISA

Supplier Data

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean NRP2 concentration was determined to be 22.87 ng/mL with a range of 17.29 - 33.43 ng/mL.

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)
  • sELISA

Supplier Data

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)

Example of human NRP2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)
  • sELISA

Supplier Data

Sandwich ELISA - Human NRP2 ELISA Kit - Extracellular (AB315040)

Interpolated concentration of native NRP2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% U-87 MG cell culture supernatant and CSF.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in cell culture media.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant, Citrate plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18.39 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NRP2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NRP2 protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Cell culture supernatant, Cerebral Spinal Fluid. Quantitate Human NRP2 with 18.39 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
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Recovery

[ { "sample": "Serum", "range": "77 - 111 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "89 - 135 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "70 - 105 %", "average": "= 93" }, { "sample": "Cerebral Spinal Fluid", "range": "82 - 128 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "75 - 117 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "106 - 128 %", "average": "= 113" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRP2 also known as Neuropilin-2 is a receptor protein involved in developmental processes including axon guidance and angiogenesis. The molecular mass of NRP2 is approximately 120 kDa. It is expressed in various tissues such as the nervous system and the vascular system but its expression can also be found in certain immune cells. NRP2 plays an important role in cellular signaling by binding to semaphorins and vascular endothelial growth factors (VEGF).
Biological function summary

NRP2 influences several processes including axonal guidance and the formation of blood vessels. It acts as a co-receptor by forming complexes with other proteins to mediate cellular responses. This role in signaling helps guide neuron growth and regulates vascular development. The NRP2-associated complexes often modulate responses to external signals contributing to the precise control of network formation in tissues.

Pathways

NRP2 plays a significant role in the VEGF signaling pathway and the semaphorin signaling pathway. These pathways are essential for cell growth migration and differentiation. NRP2 works closely with VEGF receptors like VEGFR2 to promote angiogenic responses. It also interacts with semaphorin family proteins to guide neuronal development. Together these pathways ensure the proper formation and function of neural and vascular networks.

NRP2 is connected to cancer progression and cardiovascular diseases. Altered expression of NRP2 has been observed in various cancer types where it may contribute to tumor growth and metastasis. In these diseases NRP2's interaction with its partners such as VEGFR2 becomes dysregulated affecting normal cellular processes. Cardiovascular conditions can also arise from issues in NRP2-related signaling where the normal function of blood vessel formation is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.. (Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells.
See full target information NRP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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