Human NT-proBNP ELISA Kit ab263877 is a single-wash 90-min SimpleStep ELISA used to quantify Human NT-proBNP with a sensitivity of 11.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human NT-proBNP sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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- Pakistan journal of medical sciences 39:972-9772023Expression and clinical significance of NRLP1 in patients with ST-segment elevation myocardial infarction combined with malignant ventricular arrhythmia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiXin Xie,HuiQiong Jiang,ZiLin Gao,YongJun Lin,NaJiao HongPubMed 37492291
- Molecules (Basel, Switzerland) 28:2023Phenolics Extracted from Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUrooj Umar,Sibtain Ahmed,Asra Iftikhar,Maryam Iftikhar,Wafa Majeed,Atika Liaqat,Sana Shahzad,Mateen Abbas,Tahir Mehmood,Farooq AnwarPubMed 37513325
- Biosensors 13:2023LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria António,Rui Vitorino,Ana L Daniel-da-SilvaPubMed 37504134
- Journal of cardiovascular translational research 15:381-3902021Role of Anti-Beta-1-Adrenergic Receptor Antibodies in Cardiac Dysfunction in Patients with Cirrhotic Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLixia Ma,Xiaohui Liu,Qingshan Wu,Xing Hu,Hongqun Liu,Jing Zhang,Samuel S LeePubMed 34417673
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.9 - 1400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 11.5 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:1672777, PubMed:17372040, PubMed:1914098, PubMed:9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:1672777, PubMed:17349887, PubMed:17372040, PubMed:21098034, PubMed:25339504, PubMed:9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:1914098, PubMed:9458824). Binds the clearance receptor NPR3 (PubMed:16870210). NT-proBNP. May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040). BNP(3-32). May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).
Alternative names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB
What's included?
Recommended products
Human NT-proBNP ELISA Kit ab263877 is a single-wash 90-min SimpleStep ELISA used to quantify Human NT-proBNP with a sensitivity of 11.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human NT-proBNP sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.9 - 1400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 11.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 84 - 94 % |
Sample type EDTA Plasma | Average % = 89 | Range 88 - 90 % |
Sample type Heparin Plasma | Average % = 89 | Range 84 - 93 % |
Sample type Citrate plasma | Average % = 90 | Range 85 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human NT-proBNP ELISA Kit (ab263877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NT-proBNP protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NT-proBNP with 11.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
NT-proBNP, or Brain Natriuretic Peptide, is a hormone expressed by cardiomyocytes by the gene NPPB. NT-proBNP is synthesized as a 134- amino acid preprohormone (prepro-BNP), subsequent removal of a 25 amino acid signal peptide forms pro-BNP, which is stored intracellularly. Pro-BNP is secreted as an inactive prohormone and cleavage by furin or corin forms NT-pro-BNP and the active 32- amino acid BNP-32 peptide. BNP may function as a paracrine antifibrotic factor in the heart, as well as have a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. The antibody pair in this product detect NT-pro-BNP.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NT-proBNP known as N-terminal prohormone of brain natriuretic peptide is a non-active prohormone that measures approximately 8.5 kDa in mass. This prohormone emerges from the cleavage of pro-BNP in cardiomyocytes specifically in the heart ventricles. NT-proBNP levels rise in response to ventricular volume expansion and pressure overload therefore serving as an important indicator of cardiac function. Researchers regularly utilize assays like NT-proBNP ELISA and NT-proBNP monoclonal antibodies for detecting its presence in various biological samples.
- Biological function summary
NT-proBNP stands important for cardiovascular homeostasis. It does not participate as part of a complex but plays a role on its own. When released into circulation NT-proBNP functions as an indicator of heart performance indirectly reflecting the activity of the active hormone BNP. Blood vessels dilate under BNP influence reducing systemic vascular resistance and central venous pressure which aids in heart function improvement.
- Pathways
NT-proBNP interacts significantly within the cardiac stress response pathways. It plays a focus within the natriuretic peptide system interacting with proteins such as BNP and ANP that help regulate fluid balance and blood pressure. These components together influence pathways that modulate hemodynamic stress and sodium excretion ensuring cardiovascular stability under stress conditions.
- Associated diseases and disorders
Clinicians use NT-proBNP as a biomarker for heart failure and ischemic heart disease. Elevated levels of NT-proBNP correlate with cardiac stress indicating unmet cardiac workload or myocardial ischemia. In these contexts NT-proBNP serves alongside BNP as an indicator of increased heart strain thereby offering insights into disease progression and patient response to therapeutic interventions.
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4 product images
Sandwich ELISA - Human NT-proBNP ELISA Kit (ab263877)
Example of human NT-proBNP standard curve in Sample Diluent 50BS.
The NT-proBNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human NT-proBNP ELISA Kit (ab263877)
Interpolated concentrations of native NT-proBNP in human congestive heart failure serum samples.
The concentrations of NT-proBNP were measured in duplicates, interpolated from the NT-proBNP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 16,839 pg/mL in serum.
Sandwich ELISA - Human NT-proBNP ELISA Kit (ab263877)
Serum from eight individual healthy human female donors was measured in duplicate.
Two were positive for Human BNP. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 159.7 pg/mL with a range of 76.1 – 243.3 pg/mL.
Sandwich ELISA - Human NT-proBNP ELISA Kit (ab263877)
Example of human NT-proBNP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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