AB263877

Human NT-proBNP ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Human NT-proBNP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NT-proBNP with a sensitivity of 11.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

5 Images

ELISA - Human NT-proBNP ELISA Kit (AB263877)

ELISA

Supplier Data

ELISA - Human NT-proBNP ELISA Kit (AB263877)

sELISA

Supplier Data

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Interpolated concentrations of native NT-proBNP in human congestive heart failure serum samples.

The concentrations of NT-proBNP were measured in duplicates, interpolated from the NT-proBNP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 16,839 pg/mL in serum.

sELISA

Supplier Data

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Serum from eight individual healthy human female donors was measured in duplicate.

Two were positive for Human BNP. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 159.7 pg/mL with a range of 76.1 – 243.3 pg/mL.

sELISA

Lab

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Example of human NT-proBNP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Example of human NT-proBNP standard curve in Sample Diluent 50BS.

The NT-proBNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.5 pg/mL

Range

21.9 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NT-proBNP ELISA Kit ab263877 is a rapid single-wash 90-min sandwich ELISA kit to measure Human NT-proBNP in Serum, and Plasma (Citrate, EDTA, Heparin). This Simplestep sensitivity is 11.5 pg/mL.

How the assay works

Human NT-proBNP SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

The antibodies in this assay were found not to react with the BNP-32 peptide sequence.

Human NT-proBNP ELISA Kit ab263877 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using our Human NT-proBNP ELISA Kit ab263877

Human NT-proBNP ELISA Kit ab263877 has been used to study diabetes ⁽¹⁾ and heart disease ⁽²⁾.

References:

⁽¹⁾ U. Umar, et.al., 2023, PMID: 37513325
⁽²⁾ S. Xie, et.al., 2023, PMID: 37492291

Related and Recommended products

Human NT-proBNP ELISA Kit ab263877 is commonly used to measure NT-proBNP as a marker of cardiotoxicity.

Other ELISA kits to measure cardiotoxicity biomarkers include:

- Human NT-ProANP ELISA Kit ab245705
- Human Cardiac Troponin I ELISA Kit ab200016
- Human Cardiac Troponin T ELISA Kit ab223860
- Human MYBPC3 ELISA Kit ab323737

NT-proBNP, or Brain Natriuretic Peptide, is a hormone expressed by cardiomyocytes by the gene NPPB. NT-proBNP is synthesized as a 134- amino acid preprohormone (prepro-BNP), subsequent removal of a 25 amino acid signal peptide forms pro-BNP, which is stored intracellularly. Pro-BNP is secreted as an inactive prohormone and cleavage by furin or corin forms NT-pro-BNP and the active 32- amino acid BNP-32 peptide. BNP may function as a paracrine antifibrotic factor in the heart, as well as have a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. The antibody pair in this product detect NT-pro-BNP.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 90 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "84 - 93 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NT-proBNP known as N-terminal prohormone of brain natriuretic peptide is a non-active prohormone that measures approximately 8.5 kDa in mass. This prohormone emerges from the cleavage of pro-BNP in cardiomyocytes specifically in the heart ventricles. NT-proBNP levels rise in response to ventricular volume expansion and pressure overload therefore serving as an important indicator of cardiac function. Researchers regularly utilize assays like NT-proBNP ELISA and NT-proBNP monoclonal antibodies for detecting its presence in various biological samples.

Biological function summary

NT-proBNP stands important for cardiovascular homeostasis. It does not participate as part of a complex but plays a role on its own. When released into circulation NT-proBNP functions as an indicator of heart performance indirectly reflecting the activity of the active hormone BNP. Blood vessels dilate under BNP influence reducing systemic vascular resistance and central venous pressure which aids in heart function improvement.

Pathways

NT-proBNP interacts significantly within the cardiac stress response pathways. It plays a focus within the natriuretic peptide system interacting with proteins such as BNP and ANP that help regulate fluid balance and blood pressure. These components together influence pathways that modulate hemodynamic stress and sodium excretion ensuring cardiovascular stability under stress conditions.

Clinicians use NT-proBNP as a biomarker for heart failure and ischemic heart disease. Elevated levels of NT-proBNP correlate with cardiac stress indicating unmet cardiac workload or myocardial ischemia. In these contexts NT-proBNP serves alongside BNP as an indicator of increased heart strain thereby offering insights into disease progression and patient response to therapeutic interventions.

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Product protocols

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Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).

See full target information NPPB

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

British journal of anaesthesia 132:857-866 PubMed38341283

2024

Preoperative N-terminal pro-B-type natriuretic peptide and myocardial injury after stopping or continuing renin-angiotensin system inhibitors in noncardiac surgery: a prespecified analysis of a phase 2 randomised controlled multicentre trial.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Gutierrez Del Arroyo,Akshaykumar Patel,Tom E F Abbott,Salma Begum,Priyanthi Dias,Sameer Somanath,Alexander Middleditch,Stuart Cleland,David Brealey,Rupert M Pearse,Gareth L Ackland

Frontiers in cardiovascular medicine 11:1232269 PubMed38322766

2024

Combinatorial immune checkpoint blockade increases myocardial expression of NLRP-3 and secretion of H-FABP, NT-Pro-BNP, interleukin-1β and interleukin-6: biochemical implications in cardio-immuno-oncology.

Applications

Unspecified application

Species

Unspecified reactive species

V Quagliariello,M Passariello,I Bisceglia,A Paccone,A Inno,C Maurea,R Rapuano Lembo,L Manna,M Iovine,M L Canale,M Scherillo,P A Ascierto,D Gabrielli,C De Lorenzo,N Maurea

ACS nano 17:19952-19960 PubMed37824510

2023

Amphiphilic Particle-Stabilized Nanoliter Droplet Reactors with a Multimodal Portable Reader for Distributive Biomarker Quantification.

Applications

Unspecified application

Species

Unspecified reactive species

Vishwesh Shah,Xilin Yang,Alyssa Arnheim,Shreya Udani,Derek Tseng,Yi Luo,Mengxing Ouyang,Ghulam Destgeer,Omai B Garner,Hatice C Koydemir,Aydogan Ozcan,Dino Di Carlo

Pakistan journal of medical sciences 39:972-977 PubMed37492291

2023

Expression and clinical significance of NRLP1 in patients with ST-segment elevation myocardial infarction combined with malignant ventricular arrhythmia.

Applications

Unspecified application

Species

Unspecified reactive species

SiXin Xie,HuiQiong Jiang,ZiLin Gao,YongJun Lin,NaJiao Hong

Molecules (Basel, Switzerland) 28: PubMed37513325

2023

Phenolics Extracted from Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Urooj Umar,Sibtain Ahmed,Asra Iftikhar,Maryam Iftikhar,Wafa Majeed,Atika Liaqat,Sana Shahzad,Mateen Abbas,Tahir Mehmood,Farooq Anwar

Biosensors 13: PubMed37504134

2023

LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP.

Applications

Unspecified application

Species

Unspecified reactive species

Maria António,Rui Vitorino,Ana L Daniel-da-Silva

Journal of cardiovascular translational research 15:381-390 PubMed34417673

2021

Role of Anti-Beta-1-Adrenergic Receptor Antibodies in Cardiac Dysfunction in Patients with Cirrhotic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Ma,Xiaohui Liu,Qingshan Wu,Xing Hu,Hongqun Liu,Jing Zhang,Samuel S Lee

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human NT-proBNP ELISA Kit

ELISA - Human NT-proBNP ELISA Kit (AB263877)

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Sandwich ELISA - Human NT-proBNP ELISA Kit (AB263877)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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