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AB326386

Human NT-proBNP ELISA Kit, Chemiluminescent

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Human NT-proBNP ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human NT-proBNP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.91 - 2000 pg/ml with a sensitivity of 0.82pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

5 Images
Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)
  • sELISA

Lab

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)
  • sELISA

Lab

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)

Serum of nine individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration of detected samples was determined to be 10.9 pg/mL with a range of undetected – 20.6 pg/mL.

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)
  • sELISA

Lab

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)

Dynamic range comparison between the Chemiluminescent Human NT-proBNP SimpleStep ELISA, colorimetric Human NT-proBNP SimpleStep ELISA (ab263877), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)
  • sELISA

Lab

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)

Standard curve comparison between the Chemiluminescent Human NT-proBNP SimpleStep ELISA and colorimetric Human NT-proBNP SimpleStep ELISA (ab263877) in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)
  • sELISA

Supplier Data

Sandwich ELISA - Human NT-proBNP ELISA Kit, Chemiluminescent (AB326386)

Interpolated concentrations of native NT-proBNP in human congestive heart failure serum samples.

The concentrations of NT-proBNP were measured in duplicates interpolated from the NT-proBNP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean NT-proBNP concentration was determined to be 16839 pg/mL in serum.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.82 pg/mL

Range

0.91 - 2000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human NT-proBNP ELISA Kit, Chemiluminescent (ab326386) is a rapid single-wash sandwich 55-min ELISA to measure Human NT-proBNP in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.82 pg/mL.

How the assay works

Human NT-proBNP ELISA Kit, Chemiluminescent (ab326386) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human NT-proBNP (ab326386) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NT-proBNP antibody [EPR20260-209] - BSA and Aside free (Capture) ab259657
- Anti-NT-proBNP antibody [EPR20260-85] - BSA and Azide free (Detector) ab267459
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 90 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "84 - 93 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 90" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).
See full target information NPPB
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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