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AB313904

Human NUCB2 ELISA Kit

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Human NUCB2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NUCB2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

NEFA, NUCB2, Nucleobindin-2, DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Prepronesfatin

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.25 ng/mL

Range

0.25 - 60 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NUCB2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NUCB2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human NUCB2 coated on a 96- well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP4/RAET1E, UNC5H3, VAMP-1, VAMP-2.

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Recovery

[ { "sample": "Serum", "range": "72 - 132 %", "average": "= 103.2" }, { "sample": "Plasma", "range": "119 - 146 %", "average": "= 134.5" }, { "sample": "Cell culture media", "range": "91 - 148 %", "average": "= 119.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313904-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313904-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human NUCB2 Antibody-coated ELISA Plate", "number":"AB313904-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313904-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB313904-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human NUCB2 Detection Antibody", "number":"AB313904-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313904-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313904-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human NUCB2 Protein Standard", "number":"AB313904-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUCB2 also known as nesfatin-1 precursor is a calcium-binding protein involved in various physiological processes. This 50-kDa protein integrates signals related to energy balance and is important for appetite regulation. NUCB2 is expressed in several tissues such as the hypothalamus pancreas and adipose tissue which suggests its involvement in widespread bodily functions. The protein is produced as an 82-kDa precursor protein cleaved to release active fragments like nesfatin-1 which possesses biological activity.
Biological function summary

This protein influences food intake and energy homeostasis. NUCB2 is part of a signaling complex that modulates cellular responses to metabolic needs. It functions as a satiety molecule signaling when the body does not require additional food intake. The protein's regulatory role in appetite makes it important for maintaining body weight and energy utilization. NUCB2’s expression in specific brain areas particularly indicates its role in central nervous system pathways that control energy use.

Pathways

NUCB2 engages in vital neural circuits governing appetite and energy expenditure. It connects with the melanocortin and neuropeptide Y pathways which are central to maintaining energy balance and body weight. The interaction of NUCB2 with neuropeptide Y an essential regulator of hunger exemplifies how it modulates these pathways despite its main role not being in leptin signaling.

Disturbances in NUCB2 are linked to obesity and stress-related conditions. Obesity involves disrupted signaling where NUCB2’s role as a satiety signal becomes critical. The protein also plays a part in stress-related pathways influencing stress-induced appetite changes which can relate to conditions like depression. Alterations in NUCB2 expression or function can lead to abnormal body weight and stress responses making it a potential target for therapeutic interventions in these conditions.
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Product protocols

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Target data

Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.
See full target information NUCB2
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