JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283968

Human Nucleoside Diphosphate Kinase D ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Nucleoside Diphosphate Kinase D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Nucleoside Diphosphate Kinase D in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

NM23D, NME4, NDK, Nucleoside diphosphate kinase D, nm23-H4, NDPKD

1 Images
Sandwich ELISA - Human Nucleoside Diphosphate Kinase D ELISA Kit (AB283968)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nucleoside Diphosphate Kinase D ELISA Kit (AB283968)

Example of Human Nucleoside Diphosphate Kinase D standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.34 ng/mL

Range

0.75 - 24 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human Nucleoside Diphosphate Kinase D ELISA Kit (ab283968) is designed for detection of Nucleoside Diphosphate Kinase D (NDPKD) in human plasma, serum, cell culture, and cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human NDPKD in approximately 5 hours. A polyclonal antibody specific for human NDPKD has been pre-coated onto a 96-well microplate with removable strips. NDPKD in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human NDPKD, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283968-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283968-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283968-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283968-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Nucleoside Diphosphate Kinase D Antibody", "number":"AB283968-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Nucleoside Diphosphate Kinase D Standard", "number":"AB283968-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283968-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283968-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Nucleoside Diphosphate Kinase D Microplate", "number":"AB283968-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283968-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283968-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NME4 also known as nucleoside diphosphate kinase D is a mitochondrial protein with a mass of approximately 17 kDa. It is found in the inner mitochondrial membrane and is part of the NDP (nucleoside diphosphate) kinase family. This protein plays a role in transferring gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates facilitating essential cellular energy transactions. NME4's catalytic activity helps maintain the balance and availability of nucleotides within the mitochondria.
Biological function summary

Nucleoside diphosphate kinase activity of NME4 contributes to energy homeostasis in cells. It assists in the generation of nucleoside triphosphates necessary for mitochondrial function and integrity. NME4 operates as part of multimeric complexes within the mitochondria ensuring efficient energy metabolism. Its role extends to contributing to cellular signaling pathways by providing the necessary energy substrates for a variety of cellular responses and activities.

Pathways

Researchers have found that NME4 is involved in the regulation of the mitochondrial apoptotic pathway. It interacts with other proteins involved in apoptosis such as Bcl-2 family members by influencing the mitochondrial outer membrane permeability. Additionally NME4 participates in pathways related to oxidative phosphorylation working alongside other proteins like ATP synthase to optimize energy production and utilization. These interactions underpin its versatility in sustaining cellular energy requirements and influencing cell survival.

Dysfunction in NME4 activity has been linked to mitochondrial diseases and cancer. In particular alterations in NME4 have implications in mitochondrial myopathy where energy production becomes compromised. Similarly cancer studies have shown changes in NME4 expression affect tumor growth and survival often in conjunction with proteins involved in metabolic regulation like p53. Understanding NME4's interactions and modifications can provide insights into its role in these diseases and potential therapeutic targets.
style
left-column

Product protocols

style
left-column

Target data

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed : 10799505). Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed : 18635542, PubMed : 23150663). Acts as a mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed : 18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed : 24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed : 17028143, PubMed : 18635542, PubMed : 23150663).
See full target information NME4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB228005

Anti-NME4 antibody

Western blot - Anti-NME4 antibody (AB228005)

  • 0
  • 0
Proteins & Peptides

AB211319

Recombinant human NME4 protein

SDS-PAGE - Recombinant human NME4 protein (AB211319)

  • 0
  • 0
Cell Lines & Lysates

AB258536

Human NME4 knockout HEK-293T cell lysate

Sanger Sequencing - Human NME4 knockout HEK-293T cell lysate (AB258536)

  • 0
  • 0
Cell Lines & Lysates

AB267281

Human NME4 knockout HEK-293T cell line

Sanger Sequencing - Human NME4 knockout HEK-293T cell line (AB267281)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com