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AB267594

Human OB Cadherin ELISA Kit

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Human OB Cadherin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human OB Cadherin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin, CDH11

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Sandwich ELISA - Human OB Cadherin ELISA Kit (AB267594)
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Supplier Data

Sandwich ELISA - Human OB Cadherin ELISA Kit (AB267594)

Human OB Cadherin ELISA kit (ab267594) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.8 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human OB Cadherin ELISA Kit is designed for the quantitative determination of OB Cadherin in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human OB Cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and OB Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OB Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OB Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects human OB Cadherin, no cross-reativity with Cadherin-4, 6, 8, 12, 13, 17, E-Cadherin, N-Cadherin, P-Cadherin, or VE-Cadherin is observed.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 100 %", "average": "= 93.61" }, { "sample": "Plasma", "range": "79 - 91 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "102 - 128 %", "average": "= 114" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267594-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267594-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267594-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human OB Cadherin coated Microplate (12 x 8 wells)", "number":"AB267594-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OB Cadherin Standard (Lyophilized)", "number":"AB267594-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267594-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267594-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human OB Cadherin Detection Antibody", "number":"AB267594-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267594-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267594-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OB Cadherin is a calcium-dependent cell adhesion molecule also known as Cadherin-11. It has a mass of about 120 kDa. OB Cadherin is expressed widely in mesenchymal tissues including osteoblasts fibroblasts and synovial tissues. This cadherin plays a role in the regulation of cell-cell adhesion influencing the mechanical structure and integrity of tissues. Its expression in differentiated cells indicates its involvement in tissue maintenance and repair. OB Cadherin is also contributing to the segregation of different cell types during developmental processes.
Biological function summary

OB Cadherin plays an important role in tissue morphogenesis and remodeling. It is a part of the adherens junction complex which also includes catenins. This complex mediates cell contact and communication essential for structural integrity and signaling. OB Cadherin supports the proper organization and separation of cells within tissues impacting processes like bone development and wound healing. It acts as a mediator in mechanical signaling influencing cellular responses to mechanical stress.

Pathways

OB Cadherin participates in signaling pathways involved in cell adhesion and migration notably in the Wnt and BMP pathways. These pathways are essential for developmental processes and maintain tissue homeostasis. OB Cadherin interacts closely with proteins like beta-catenin playing a role in transcriptional regulation that influences cell fate decisions. Its function in these pathways demonstrates its critical role in regulating cellular activities tied to growth and differentiation.

OB Cadherin is linked to conditions like rheumatoid arthritis and cancer metastasis. In rheumatoid arthritis overexpression of OB Cadherin in synovial tissues can contribute to joint inflammation and tissue destruction associated with interactions with fibroblast-like synoviocytes. In cancer metastasis OB Cadherin's role in cell adhesion and migration highlights its potential influence in the spread of cancer cells often in relation to altered interactions with proteins like N-cadherin. These associations make OB Cadherin an important target for research into disease mechanisms and therapies.
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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Required for proper focal adhesion assembly (PubMed : 33811546). Involved in the regulation of cell migration (PubMed : 33811546).
See full target information CDH11
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