Human Obestatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Obestatin with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed:10604470). Induces the release of growth hormone from the pituitary (PubMed:10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed:10604470). Induces the release of growth hormone from the pituitary (PubMed:10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
Alternative names
MTLRP, UNQ524/PRO1066, GHRL, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46
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Human Obestatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Obestatin with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type EDTA Plasma | Average % = 96 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Obestatin ELISA Kit (ab264628) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Obestatin protein in edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Obestatin with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to amino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Obestatin also known as the obestatin hormone is a 23-amino acid peptide with a mass of approximately 2.5 kDa. Researchers discovered it as a cleavage product from the Ghrelin preprohormone. Obestatin is mainly expressed in the stomach and small intestine but one can also find it in other tissues such as the pancreas and adipose tissue. This peptide acts mechanically by binding to its receptor GPR39 although the binding affinity and downstream effects remain subjects of investigation.
- Biological function summary
This peptide hormone interacts with several bodily processes mostly related to gastrointestinal functions and energy homeostasis. It does not form part of a larger protein complex but acts independently. Studies suggest it could play roles in reducing food intake and slowing down gastric emptying potentially contrasting the action of Ghrelin which stimulates hunger. Understanding its precise biological role remains complex with ongoing research needed to clarify its function fully.
- Pathways
Several metabolic circuits include obestatin within their framework. It engages in pathways associated with energy balance and gut motility. In relation to the ghrelin axis specialists evaluate its potential modulatory effects given its origin from the same precursor. So far research indicates possible interplay with pathways influencing insulin release although the mechanistic details are not yet fully understood.
- Associated diseases and disorders
Preliminary research indicates obestatin might have connections to obesity and metabolic syndrome. Some scholars propose a potential protective effect against weight gain but findings often vary. Obestatin's relationship with the aforementioned diseases might involve its interaction with ghrelin highlighting a complex regulatory network that warrants further investigation. These insights could lead to novel therapeutic avenues for tackling metabolic disorders.
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2 product images
Sandwich ELISA - Human Obestatin ELISA Kit (ab264628)
Example of human Obestatin standard curve in Sample Diluent NS.
The Obestatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Obestatin ELISA Kit (ab264628)
Interpolated concentrations of native Obestatin in human plasma (EDTA) samples.
The concentrations of Obestatin were measured in duplicates, interpolated from the Obestatin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Obestatin concentration was determined to be 289 pg/mL in plasma (EDTA).
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