AB269546

Human OGA ELISA Kit

Recombinant
SimpleStep
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Human OGA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human OGA with a sensitivity of 0.4 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HEXC, KIAA0679, MEA5, MGEA5, OGA, Protein O-GlcNAcase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Beta-hexosaminidase, Meningioma-expressed antigen 5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, NCOAT

3 Images

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

sELISA

Supplier Data

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Representative image of Human OGA ELISA Kit (ab269546)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human OGA Capture Antibody

- 10X Human OGA Detector Antibody

- Human OGA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Interpolated concentrations of native OGA in human SH-SY5Y cell extract based on a 200 μg/mL extract load.

The concentrations of OGA were measured in duplicate and interpolated from the OGA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Example of human OGA standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The OGA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.4 ng/mL

Range

1.2 - 75 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human OGA ELISA Kit (ab269546) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OGA protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OGA with 0.4 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Protein O-GlcNAcase (OGA, NAG), also referred to as Beta-N-Acetylhexosaminidase and Meningioma-expressed antigen 5 (MGEA5) is a 916 amino acid enzyme encoded by the gene *OGA*. OGA catalyzes the hydrolysis of GlcNac (N-acetyl-Beta-D-glucosamine) from O-glycosylated serine or threonine residues to form free N-acetyl-D-glucosamine generally in the cytosol. Thiazoline derivatives of GlcNAc have been found to act as OGA enzyme inhibitors by mimicking the reaction intermediates. OGA has a broad range of proteins that act as substrates, which may modulate downstream effects such as transcription and proteomic activities. OGA plays a role in embryonic development, brain activity, and hormone production.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "81 - 90 %", "average": "= 84" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269546-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269546-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB269546-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269546-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269546-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OGA Detector Antibody", "number":"AB269546-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269546-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269546-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB269546-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB269546-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269546-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OGA Capture Antibody", "number":"AB269546-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OGA Lyophilized Recombinant Protein", "number":"AB269546-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGEA5 also referred to as OGA or O-GlcNAcase serves an important mechanical role in cellular metabolism. This enzyme with a molecular mass of around 103 kDa is primarily involved in the removal of O-GlcNAc modifications from serine/threonine residues on nuclear and cytoplasmic proteins. It is expressed in various tissues but exhibits predominant activity in the brain liver and pancreas. Alternative names like "OGA stains" or "OGA b" may also be encountered in literature or research contexts.

Biological function summary

MGEA5 acts as an important modulator of protein function and regulation by cycling O-GlcNAc modifications thereby influencing processes like transcription signal transduction and apoptosis. While not part of a well-defined complex it frequently interacts with other proteins involved in glucose metabolism and the cellular stress response. Its activity works in concert with OGT (O-GlcNAc transferase) which adds O-GlcNAc maintaining a balance important for cellular function.

Pathways

MGEA5 involvement centers largely around glucose homeostasis and the insulin signaling pathway. It modulates the function of proteins like insulin receptor substrate (IRS) and other key regulators in this pathway affecting metabolic regulation and energy homeostasis. It shows a direct relationship with OGT as they reciprocate in action effects highlighting their collaborative role in cellular pathways that affect cellular signaling and survival.

MGEA5 has been implicated in neurodegenerative diseases and diabetes. In Alzheimer's disease altered O-GlcNAc cycling affects tau protein homeostasis which is significant in disease pathogenesis. Likewise in diabetes MGEA5 influences insulin resistance by regulating O-GlcNAc levels on insulin signaling molecules. Dysregulation of these processes may also involve proteins such as beta-amyloid further linking MGEA5 to these pathological states.

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Product protocols

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Target data

Isoform 1. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins (PubMed : 11148210, PubMed : 11788610, PubMed : 20673219, PubMed : 22365600, PubMed : 24088714, PubMed : 28939839, PubMed : 37962578). Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1 (PubMed : 28939839, PubMed : 37962578). Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed : 20673219). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed : 24088714).. Isoform 3. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

See full target information OGA

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human OGA ELISA Kit

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Sandwich ELISA - Human OGA ELISA Kit (AB269546)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise