JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277477

Human OLFM2 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human OLFM2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human OLFM2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NOE2, OLFM2, Noelin-2, Olfactomedin-2

1 Images
ELISA - Human OLFM2 ELISA Kit (AB277477)
  • ELISA

Supplier Data

ELISA - Human OLFM2 ELISA Kit (AB277477)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.45 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human OLFM2 ELISA Kit (ab277477) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human OLFM2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human OLFM2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OLFM2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OLFM2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OLFM2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "79 - 124 %", "average": "= 102.1" }, { "sample": "Plasma", "range": "69 - 94 %", "average": "= 79.98" }, { "sample": "Cell culture media", "range": "99 - 141 %", "average": "= 121.4" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277477-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277477-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277477-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human OLFM2 coated Microplate", "number":"AB277477-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human OLFM2 Antibody", "number":"AB277477-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB277477-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OLFM2 standard protein (Lyophilized)", "number":"AB277477-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277477-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277477-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Olfactomedin 2 (OLFM2) also known as Noelin 2 is a protein encoded by the OLFM2 gene. It has a mass of approximately 60 kDa. OLFM2 localizes primarily to the extracellular matrix and the cytosol. Expression of this protein occurs in several tissues particularly in the nervous system and ocular tissues where it likely plays a role in the cellular makeup and maintenance.
Biological function summary

OLFM2 is involved in neural development and neuroprotection. It often functions as part of larger protein complexes facilitating cell-cell interactions and signal transduction processes. Additionally the protein influences axon guidance and synaptic organization contributing to the development and integrity of neural networks.

Pathways

OLFM2 integrates into various signaling pathways including the Wnt signaling pathway and the TGF-beta signaling pathway. These pathways regulate important processes like cell proliferation and differentiation. The protein often associates with other components like beta-catenin in the Wnt pathway reflecting its involvement in maintaining cellular homeostasis.

OLFM2 has links to conditions such as glaucoma and retinitis pigmentosa. In these cases OLFM2 interacts with proteins like myocilin which play roles in the pathology of these disorders. The disruption in OLFM2 function or expression can lead to altered cellular and molecular processes contributing to the disease's development and progression.
style
left-column

Product protocols

style
left-column

Target data

Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and translocation of OLFM2 to the nucleus where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (PubMed : 25298399). Plays a role in AMPAR complex organization (By similarity). Is a regulator of vascular smooth-muscle cell (SMC) phenotypic switching, that acts by promoting RUNX2 and inhibiting MYOCD binding to SRF. SMC phenotypic switching is the process through which vascular SMCs undergo transition between a quiescent contractile phenotype and a proliferative synthetic phenotype in response to pathological stimuli. SMC phenotypic plasticity is essential for vascular development and remodeling (By similarity).
See full target information OLFM2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com