AB267805

Human OLFM4 ELISA Kit

Recombinant
SimpleStep
What is this?

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(3 Publications)

Human OLFM4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human OLFM4 with a sensitivity of 315.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1

4 Images

ELISA - Human OLFM4 ELISA Kit (AB267805)

ELISA

Supplier Data

ELISA - Human OLFM4 ELISA Kit (AB267805)

sELISA

Supplier Data

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Interpolated concentrations of native OLFM4 in human serum and plasma samples.

The concentrations of OLFM4 were measured in duplicates, interpolated from the OLFM4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 4,710 pg/mL in serum and 4,029 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Example of human OLFM4 standard curve in Sample Diluent NS.

The OLFM4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Interpolated concentrations of native OLFM4 in human colon tissue extract based on a 400 μg/mL extract load.

The concentrations of OLFM4 were measured in duplicate and interpolated from the OLFM4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 48,973 pg/mL in colon tissue extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture media, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 315.7 pg/mL

Range

1171.88 - 75000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human OLFM4 ELISA Kit ab267805 is a rapid single-wash 90-min Sandwich ELISA to measure Human OLFM4 in cell culture media, citrate plasma, serum, tissue extracts. This SimpleStep sensitivity is 315.7 pg/mL.

How the assay works

Human OLFM4 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human OLFM4 ELISA Kit ab267805 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-OLFM4 antibody [EPR22335-74] - BSA and Azide free (Capture) ab269391
- Anti-OLFM4 antibody [EPR22335-163] - BSA and Azide free (Detector) ab269391

Olfactomedin-4 (OLFM4), formerly known as GW112 or hGC-1, is a member of the olfactomedin gene family and predominantly expressed in bone marrow, small intestine, colon, and prostrate. Studies have revealed OLFM4 to play important roles in several cellular functions such as neurogenesis, cell adhesion, cell-cycle regulation, and tumorigenesis. Moreover, studies have suggested OLFM4 to serve as a robust biomarker in studying stem cells and cancer in small intestines, stomach, and colon.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 87 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Tissue Extracts", "range": "89 - 110 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "83 - 88 %", "average": "= 86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267805-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267805-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267805-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267805-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267805-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267805-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OLFM4 Capture Antibody", "number":"AB267805-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB267805-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OLFM4 Lyophilized Recombinant Protein", "number":"AB267805-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267805-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB267805-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OLFM4 Detector Antibody", "number":"AB267805-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.

Biological function summary

OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.

Pathways

OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.

OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.

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Product protocols

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Target data

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

See full target information OLFM4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of innate immunity 15:351-364 PubMed36450268

2022

The Olfactomedin-4-Defined Human Neutrophil Subsets Differ in Proteomic Profile in Healthy Individuals and Patients with Septic Shock.

Applications

Unspecified application

Species

Unspecified reactive species

Hans Lundquist,Henrik Andersson,Michelle S Chew,Jyotirmoy Das,Maria V Turkina,Amanda Welin

Journal of inflammation research 14:7035-7051 PubMed34955649

2021

OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen Chen

Canadian journal of gastroenterology & hepatology 2021:5601678 PubMed34912753

2021

Olfm4 Is Highly Expressed in HCC Patients and as a Biomarker and Therapeutic Target for HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Wei,Qingzhu Song,Fenglan Zhang,Tian Yuan

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human OLFM4 ELISA Kit

ELISA - Human OLFM4 ELISA Kit (AB267805)

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise