Human OLFM4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human OLFM4 with a sensitivity of 315.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Publications
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- Journal of innate immunity 15:351-3642022The Olfactomedin-4-Defined Human Neutrophil Subsets Differ in Proteomic Profile in Healthy Individuals and Patients with Septic Shock.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHans Lundquist,Henrik Andersson,Michelle S Chew,Jyotirmoy Das,Maria V Turkina,Amanda WelinPubMed 36450268
- Journal of inflammation research 14:7035-70512021OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen ChenPubMed 34955649
- Canadian journal of gastroenterology & hepatology 2021:56016782021Olfm4 Is Highly Expressed in HCC Patients and as a Biomarker and Therapeutic Target for HCC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Wei,Qingzhu Song,Fenglan Zhang,Tian YuanPubMed 34912753
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1171.88 - 75000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 315.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
Alternative names
GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1
What's included?
Recommended products
Human OLFM4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human OLFM4 with a sensitivity of 315.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1171.88 - 75000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 315.7 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86 | Range 84 - 87 % |
Sample type Cell culture media | Average % = 95 | Range 94 - 97 % |
Sample type Tissue Extracts | Average % = 101 | Range 89 - 110 % |
Sample type Citrate plasma | Average % = 86 | Range 83 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human OLFM4 ELISA Kit (ab267805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OLFM4 protein in serum, cit plasma, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OLFM4 with 315.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Olfactomedin-4 (OLFM4), formerly known as GW112 or hGC-1, is a member of the olfactomedin gene family and predominantly expressed in bone marrow, small intestine, colon, and prostrate. Studies have revealed OLFM4 to play important roles in several cellular functions such as neurogenesis, cell adhesion, cell-cycle regulation, and tumorigenesis. Moreover, studies have suggested OLFM4 to serve as a robust biomarker in studying stem cells and cancer in small intestines, stomach, and colon.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.
- Biological function summary
OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.
- Pathways
OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.
- Associated diseases and disorders
OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.
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3 product images
Sandwich ELISA - Human OLFM4 ELISA Kit (ab267805)
Example of human OLFM4 standard curve in Sample Diluent NS.
The OLFM4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human OLFM4 ELISA Kit (ab267805)
Interpolated concentrations of native OLFM4 in human serum and plasma samples.
The concentrations of OLFM4 were measured in duplicates, interpolated from the OLFM4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 4,710 pg/mL in serum and 4,029 pg/mL in plasma (citrate).
Sandwich ELISA - Human OLFM4 ELISA Kit (ab267805)
Interpolated concentrations of native OLFM4 in human colon tissue extract based on a 400 μg/mL extract load.
The concentrations of OLFM4 were measured in duplicate and interpolated from the OLFM4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 48,973 pg/mL in colon tissue extract.
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