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AB326735

Human OLFM4 ELISA Kit, Chemiluminescent

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Human OLFM4 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human OLFM4 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 274.35 - 200000 pg/ml with a sensitivity of 60.1 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1

4 Images
Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)
  • sELISA

Supplier Data

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)

Interpolated concentrations of native OLFM4 in human serum and plasma samples.

The concentrations of OLFM4 were measured in duplicates, interpolated from the OLFM4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 4,710 pg/mL in serum and 4,029 pg/mL in plasma (citrate).

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)
  • sELISA

Supplier Data

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)

Interpolated concentrations of native OLFM4 in human colon tissue extract based on a 400 μg/mL extract load.

The concentrations of OLFM4 were measured in duplicate and interpolated from the OLFM4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 48,973 pg/mL in colon tissue extract.

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)
  • sELISA

Lab

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)

Standard curve comparison between the Chemiluminescent Human OLFM4 SimpleStep ELISA and colorimetric Human OLFM4 SimpleStep ELISA (ab267805) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)
  • sELISA

Lab

Sandwich ELISA - Human OLFM4 ELISA Kit, Chemiluminescent (AB326735)

Dynamic range comparison between the Chemiluminescent Human OLFM4 SimpleStep ELISA, colorimetric Human OLFM4 SimpleStep ELISA (ab267805), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, Cell culture media, Tissue Extracts, Citrate plasma

Reacts with

Human, Monkey, Cow

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 60.1 pg/mL

Range

274.35 - 200000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human OLFM4 ELISA Kit, Chemiluminescent (ab326735) is a rapid single-wash sandwich 55-min ELISA to measure Human OLFM4 in Serum, Cell culture media, Tissue Extracts, Citrate plasma with a sensitivity of 60.1 pg/mL.

How the assay works

Human OLFM4 ELISA Kit, Chemiluminescent (ab326735) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human OLFM4 (ab326735) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Olfactomedin-4 antibody [EPR22335-74] - BSA and Aside free (Capture) ab269390
- Anti-Olfactomedin-4 antibody [EPR22335-163] - BSA and Aside free (Detector) ab269391
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 87 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "94 - 97 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "89 - 110 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "83 - 88 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326735-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human OLFM4 Detector Antibody", "number":"AB326735-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326735-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human OLFM4 Capture Antibody", "number":"AB326735-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326735-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326735-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326735-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326735-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326735-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OLFM4 Lyophilized Recombinant Protein", "number":"AB326735-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326735-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326735-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
See full target information OLFM4
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com