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AB277442

Human OMD ELISA kit

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Human OMD ELISA kit is a Sandwich ELISA for the measurement of Human OMD in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SLRR2C, UNQ190/PRO216, OMD, Osteomodulin, Keratan sulfate proteoglycan osteomodulin, Osteoadherin, KSPG osteomodulin, OSAD

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ELISA - Human OMD ELISA kit (AB277442)
  • ELISA

Supplier Data

ELISA - Human OMD ELISA kit (AB277442)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.4 ng/mL

Range

1.229 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB154249

Anti-OMD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OMD antibody (AB154249)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human OMD ELISA Kit (ab277442) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human OMD in serum (human OMD concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human OMD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OMD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OMD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OMD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 101 %", "average": "= 86.79" }, { "sample": "Cell culture media", "range": "76 - 98 %", "average": "= 89.67" }, { "sample": "Plasma", "range": "67 - 77 %", "average": "= 71.72" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277442-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277442-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277442-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human OMD coated Microplate", "number":"AB277442-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human OMD standard protein (Lyophilized)", "number":"AB277442-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human OMD Antibody", "number":"AB277442-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277442-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB277442-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB277442-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277442-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB277442-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteomodulin (OMD) also referred to as osteoadherin is a small leucine-rich proteoglycan with a molecular weight of approximately 48 kDa. This protein plays a significant role in bone biology and is primarily expressed in mineralized tissues such as osteoblasts and dentin. OMD contains multiple leucine-rich repeats that facilitate its interaction with collagen fibrils suggesting a role in the regulation of collagen assembly and matrix organization.
Biological function summary

The functions of osteomodulin contribute to maintaining bone structure and integrity. It binds to type I collagen and assists in organizing the extracellular matrix in bone tissue. OMD is not part of a larger protein complex but acts individually to stabilize the mineralized matrix. Its presence is essential for proper bone mineralization and it participates in cell signaling that governs osteogenic differentiation.

Pathways

Osteomodulin has involvement in bone development and remodeling processes. It plays a part in the TGF-beta signaling pathway which is important for bone matrix production and maintenance. OMD interacts with other matrix-related proteins including decorin and biglycan through these pathways supporting structural integrity and influencing cellular responses.

Osteomodulin's dysfunction connects to bone-related pathologies like osteoarthritis and osteoporosis. Abnormal expression of OMD can disrupt collagen regulation contributing to these conditions. It may also influence interactions with other proteins involved in these diseases such as matrix metalloproteinases further affecting tissue degradation and remodeling in diseased states.
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Product protocols

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Target data

May be implicated in biomineralization processes. Has a function in binding of osteoblasts via the alpha(V)beta(3)-integrin.
See full target information OMD
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websiteProtocolBooklet
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Product promise

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