Human Omentin ELISA Kit (ITLN1) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Omentin (ITLN1) in Serum samples.
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- International journal of breast cancer 2022:59719812022Diagnostic and Prognostic Role of Serum Omentin and NGAL Levels in Egyptian Breast Cancer Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAl-Shimaa Mahmoud Abas,Mohamed H Sherif,Sara A Elmoneam FaragPubMed 36160364
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.3 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.21 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:11313366, PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed:26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed:26148048). Does not bind human glycans (PubMed:26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed:16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed:16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed:11747454, PubMed:23921499).
Alternative names
INTL, ITLN, LFR, UNQ640/PRO1270, ITLN1, Intelectin-1, ITLN-1, Endothelial lectin HL-1, Galactofuranose-binding lectin, Intestinal lactoferrin receptor, Omentin
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Human Omentin ELISA Kit (ITLN1) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Omentin (ITLN1) in Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.3 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.21 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 4 | mean - | SD - | C.V. 7.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99 | Range 80 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Omentin ELISA Kit (ITLN1) (ab269545) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Omentin (ITLN1) protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Omentin (ITLN1) with 0.21 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Omentin, also known as intelectin-1 (ITLN) and intestinal lactoferrin receptor, is an intelectin encoded by the *ITLN1* gene. Intelectins are carbohydrate-binding proteins that bind microbe-specific carbohydrate residues in a calcium-dependent manner. Omentin is expressed containing an 18- amino acid signal peptide, a C-terminal 15- amino acid pro-peptide sequence and a 280- amino acid mature chain. Omentin recognizes terminal acyclic 1,2-diol moieties, which includes lactoferrin and D-galactofuranose. Omentin does not bind to human glycans and can bind to certain gram-positive and gram-negative bacteria. Omentin has been found to be highly expressed in omental adipose tissue and in the small intestine.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ITLN1 also known as 'omentin-1' or 'omentin' is a carbohydrate-binding protein mainly involved in the recognition and binding of microbial polysaccharides. This protein weighs approximately 36 kDa and is predominantly expressed in visceral fat and other tissues such as the heart lungs and placenta. ITLN1 functions as an adipokine a signaling molecule released by fat tissue and it plays a significant role in regulating glucose metabolism and insulin sensitivity.
- Biological function summary
This target contributes to various important processes related to inflammatory and immune responses. ITLN1 is part of a complex network of interactions involving lectin receptors and other carbohydrate-binding proteins. It functions in response to pathogens by recognizing and trapping them thereby aiding in pathogen clearance. Its anti-inflammatory effects are mediated through its capacity to bind to ligands found on the surface of bacteria therefore preventing their adhesion and invasion.
- Pathways
ITLN1 interacts with the insulin signaling and inflammation pathways. It has a direct relation with the PI3K/Akt pathway known to play roles in glucose uptake and metabolism. This interaction has a connection to proteins like insulin receptor substrate-1 (IRS-1) through this pathway. Additionally ITLN1 engages with adipocytokine pathways impacting processes like lipid metabolism and inflammation.
- Associated diseases and disorders
Researchers have found links between ITLN1 and metabolic diseases particularly type 2 diabetes and obesity. Reduced levels of ITLN1 correlate with poor metabolic control and increased risk for these conditions. Moreover its expression and secretion tend to alter in response to inflammation linking it with inflammatory disorders and processes. Other proteins such as adiponectin also involve themselves in these metabolic pathways providing insight into their interconnected roles in metabolic homeostasis.
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4 product images
Sandwich ELISA - Human Omentin ELISA Kit (ITLN1) (ab269545)
Example of human Omentin standard curve in Sample Diluent NS with 1X Cell Extraction Enhancer.
The Omentin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Western blot - Human Omentin ELISA Kit (ITLN1) (ab269545)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [Py72] ab269515).
Multiple bands represent phosph-tyrosine containing proteins detected by Anti-Phosphotyrosine antibody [Py72] ab269515.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 3.25 secs; Lanes 5-6: 3 mins.
All lanes: Western blot - Anti-Phosphotyrosine antibody [Py72] (Anti-Phosphotyrosine antibody [Py72] ab269515) at 0.196 µg/mL
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 2: NIH/3T3 treated with 10mM pervanadate for 20 minutes, whole cell lysates at 20 µg
Lane 3: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg
Lane 4: Daudi treated with 10mM pervanadate for 30 minutes, whole cell lysates at 20 µg
Lane 5: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6: MCF7 treated with 1mM pervanadate for 20 minutes, whole cell lysates at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Sandwich ELISA - Human Omentin ELISA Kit (ITLN1) (ab269545)
Interpolated concentrations of native Omentin in human serum.
The concentrations of Omentin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 159.4 ng/mL in serum.
Sandwich ELISA - Human Omentin ELISA Kit (ITLN1) (ab269545)
Serum from eight individual healthy Omentin female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Omentin concentration was determined to be 190.6 ng/mL with a range of 97.4 – 311.8 ng/mL.
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