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AB326398

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent

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Human Omentin (ITLN1) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Omentin (ITLN1) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 36000 pg/ml with a sensitivity of 10.5pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

INTL, ITLN, LFR, UNQ640/PRO1270, ITLN1, Intelectin-1, ITLN-1, Endothelial lectin HL-1, Galactofuranose-binding lectin, Intestinal lactoferrin receptor, Omentin

5 Images
Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Standard curve comparison between the Chemiluminescent Human Omentin SimpleStep ELISA and colorimetric Human Omentin SimpleStep ELISA (ab269545) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Lab

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Dynamic range comparison between the Chemiluminescent Human Omentin SimpleStep ELISA, colorimetric Human Omentin SimpleStep ELISA (ab269545), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Supplier Data

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Interpolated concentrations of native Omentin in human serum.

The concentrations of Omentin were measured in duplicates interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean target concentration was determined to be 159.4 ng/mL in serum.

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)
  • sELISA

Supplier Data

Sandwich ELISA - Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (AB326398)

Serum from eight individual healthy Omentin female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Omentin concentration was determined to be 190.6 ng/mL with a range of 97.4 – 311.8 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.5 pg/mL

Range

16.46 - 36000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (ab326398) is a rapid single-wash sandwich 55-min ELISA to measure Human Omentin (ITLN1) in Serum with a sensitivity of 10.5 pg/mL.

How the assay works

Human Omentin (ITLN1) ELISA Kit, Chemiluminescent (ab326398) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Omentin (ITLN1) (ab326398) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ITLN1 antibody [EPR20311-300] - BSA and Aside free (Capture) ab252674
- Anti-ITLN1 antibody [EPR20311-224] - BSA and Aside free (Detector) ab252615
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.1" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 109 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed : 11313366, PubMed : 26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed : 26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed : 26148048). Does not bind human glycans (PubMed : 26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed : 16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed : 16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed : 11747454, PubMed : 23921499).
See full target information ITLN1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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