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AB215543

Human Oncostatin M/OSM ELISA Kit

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(1 Publication)

Human Oncostatin M/OSM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Oncostatin M/OSM with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Oncostatin-M, OSM

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Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Lab

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Example of human Oncostatin M/OSM standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Lab

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Interpolated concentration of native Oncostatin M/OSM was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% stimulated PBMC supernatant (1.5% PHA-M, 46h). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Supplier Data

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Comparison of Oncostatin M in unstimulated and PHA-M stimulated human PBMC cell supernatants.

Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Oncostatin M were measured in 25% supernatant samples in duplicates and interpolated from the Oncostatin M standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 2800 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 96 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Supplier Data

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Interpolated concentrations of native Oncostatin M in human cell culture supernatant samples.

The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated U937 supernatant 50% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean (target) concentration was determined to be 362.5 pg/mL in neat stimulated U937 supernatant and 2800 pg/mL in neat stimulated PBMC supernatant.

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Supplier Data

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Example of human Oncostatin M standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Supplier Data

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Comparison of Oncostatin M in unstimulated and TPA stimulated human U937 cell supernatants.

U937 cells were cultured in the absence or presence of 10 ng/mL TPA for 72 hours. The concentrations of Oncostatin M were measured in 50% supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 363 pg/mL in neat TPA stimulated U937 cell supernatant, 24.9 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)
  • sELISA

Supplier Data

Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (AB215543)

Interpolated concentrations of spike Oncostatin M in human serum and plasma samples.

The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.1 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Oncostatin M/OSM ELISA Kit (ab215543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oncostatin M/OSM protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Oncostatin M/OSM with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Oncostatin M is a 28-kDa pleiotropic cytokine of the IL-6 family that is a product of activated T lymphocytes, monocytes, neutrophils, and some tumor cells including breast cancer epithelial cells. Oncostatin M participates in a number of developmental, skeletal and immunological processes. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. Oncostatin M regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. It uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Oncostatin M is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "82 - 90 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "89 - 98 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "78 - 81 %", "average": "= 80" }, { "sample": "Cell culture media", "range": "88 - 114 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.
Biological function summary

Oncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.

Pathways

Oncostatin M involves itself in significant pathways like the JAK/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.

Oncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).
See full target information OSM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of cardiology. Heart & vasculature 35:100829 PubMed34235245

2021

Oncostatin M is a novel biomarker for coronary artery disease - A possibility as a screening tool of silent myocardial ischemia for diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Ikeda,Koichi Sato,Morihiko Takeda,Keita Miki,Kentaro Aizawa,Tsuyoshi Takada,Koji Fukuda,Nobuyuki Shiba
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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