Human Oncostatin M/OSM ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Oncostatin M/OSM with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).
Alternative names
Oncostatin-M, OSM
What's included?
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Human Oncostatin M/OSM ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Oncostatin M/OSM with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 5 | C.V. 8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 102 | Range 88 - 114 % |
Sample type Serum | Average % = 93 | Range 86 - 97 % |
Sample type EDTA Plasma | Average % = 85 | Range 82 - 90 % |
Sample type Heparin Plasma | Average % = 95 | Range 89 - 98 % |
Sample type Citrate plasma | Average % = 80 | Range 78 - 81 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Oncostatin M in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Oncostatin M protein in human serum, plasma, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint® SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint® HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Oncostatin M (OSM) is a 28-kDa pleiotropic cytokine of the IL-6 family that is a product of activated T lymphocytes, monocytes, neutrophils, and some tumor cells including breast cancer epithelial cells. Oncostatin M participates in a number of developmental, skeletal and immunological processes. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. Oncostatin M regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. It uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Oncostatin M is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.
- Biological function summary
Oncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.
- Pathways
Oncostatin M involves itself in significant pathways like the JAK/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.
- Associated diseases and disorders
Oncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.
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Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit, Fluorescent (ab229401)
Example of human Oncostatin M standard curve in Sample Diluent NS.
The Oncostatin M standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit, Fluorescent (ab229401)
Interpolated concentrations of native Oncostatin M in human cell culture supernatant samples.
The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated U937 supernatant 50% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean (target) concentration was determined to be 362.5 pg/mL in neat stimulated U937 supernatant and 2800 pg/mL in neat stimulated PBMC supernatant.
Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit, Fluorescent (ab229401)
Interpolated concentrations of spike Oncostatin M in human serum and plasma samples.
The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit, Fluorescent (ab229401)
Comparison of Oncostatin M in unstimulated and TPA stimulated human U937 cell supernatants.
U937 cells were cultured in the absence or presence of 10 ng/mL TPA for 72 hours. The concentrations of Oncostatin M were measured in 50% supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 363 pg/mL in neat TPA stimulated U937 cell supernatant, 24.9 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).
Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit, Fluorescent (ab229401)
Comparison of Oncostatin M in unstimulated and PHA-M stimulated human PBMC cell supernatants.
Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Oncostatin M were measured in 25% supernatant samples in duplicates and interpolated from the Oncostatin M standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 2800 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 96 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).
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