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AB193711

Human Osteoactivin ELISA Kit

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(2 Publications)

Human Osteoactivin ELISA Kit is a sandwich ELISA designed to quantify Human Osteoactivin with a sensitivity of 45 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 45 - 12000 pg/mL

View Alternative Names

HGFIN, NMB, UNQ1725/PRO9925, GPNMB, Transmembrane glycoprotein NMB, Hematopoietic growth factor inducible neurokinin-1 type

2 Images
Sandwich ELISA - Human Osteoactivin ELISA Kit (AB193711)
  • sELISA

Supplier Data

Sandwich ELISA - Human Osteoactivin ELISA Kit (AB193711)

Typical standard curve in Assay Diluent A.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Human Osteoactivin ELISA Kit (AB193711)
  • sELISA

Supplier Data

Sandwich ELISA - Human Osteoactivin ELISA Kit (AB193711)

Typical standard curve in Assay Diluent B.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

45 - 12000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Osteoactivin ELISA Kit ab193711 is a sandwich ELISA to measure Human Osteoactivin in serum, plasma, cell culture supernatant with a sensitivity of 45 pg/ml.

How the assay works

This assay employs an antibody specific for Human Osteoactivin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Osteoactivin present in the samples. The wells are washed and biotinylated anti-Human Osteoactivin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Osteoactivin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Osteoactivin ELISA Kit ab193711 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 126 %", "average": "= 117.8" }, { "sample": "Plasma", "range": "107 - 126 %", "average": "= 116.5" }, { "sample": "Cell culture media", "range": "98 - 149 %", "average": "= 112.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193711-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193711-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193711-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Osteoactivin detection antibody", "number":"AB193711-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Osteoactivin Standard (lyophilized)", "number":"AB193711-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193711-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193711-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB193711-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human Osteoactivin Microplate (12 strips x 8 wells)", "number":"AB193711-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.
Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.
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Product protocols

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Target data

Could be a melanogenic enzyme.
See full target information GPNMB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:7985 PubMed35568699

2022

Glycoprotein nonmetastatic melanoma protein B (GNMPB) as a novel biomarker for cerebral adrenoleukodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Leyla A Taghizadeh,Carina J King,David R Nascene,Ashish O Gupta,Paul J Orchard,LeeAnn Higgins,Todd W Markowski,Erin E Nolan,Justin W Furcich,Troy C Lund

International journal of molecular sciences 22: PubMed34639184

2021

GPNMB Extracellular Fragment Protects Melanocytes from Oxidative Stress by Inhibiting AKT Phosphorylation Independent of CD44.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Wang,Yasutaka Kuroda,Lingli Yang,Sylvia Lai,Yukiko Mizutani,Arunasiri Iddamalgoda,Jiao Guo,Asako Yamamoto,Daiki Murase,Yoshito Takahashi,Leihong Xiang,Shintaro Inoue,Daisuke Tsuruta,Ichiro Katayama
View all publications
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websiteProtocolBooklet
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