Human Osteocalcin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteocalcin with a sensitivity of 13.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Images
Publications
Filter by
- PloS one 17:e02763282022Xianling Gubao attenuates high glucose-induced bone metabolism disorder in MG63 osteoblast-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinlong Chen,Yan Li,Zhongwen Zhang,Liping Chen,Yaqian Liu,Shuhong Huang,Xiaoqian ZhangPubMed 36548302
- Computational and mathematical methods in medicine 2022:90890842022Effect of nHA-Coated Femoral Stem Prosthesis Combined with Platelet-Rich Plasma in Hemi Hip Replacement of Femoral Neck Fracture in the Elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Yang,Dabin Wu,Tongsheng Liu,Guozheng FangPubMed 35991151
- Materials (Basel, Switzerland) 15:2022Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Fernández-Lizárraga,Julieta García-López,Sandra E Rodil,Rosa María Ribas-Aparicio,Phaedra Silva-BermudezPubMed 35955174
- Pharmaceutics 14:2022Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Giannasi,Laura Mangiavini,Stefania Niada,Andrea Colombo,Elena Della Morte,Valeria Vismara,Andrea Ambrosanio,Paolo Savadori,Sara Casati,Giuseppe M Peretti,Anna Teresa BriniPubMed 35745803
- Journal of musculoskeletal & neuronal interactions 22:242-2502022Foxf1 gene increases the risk of osteoporosis in rats by inhibiting osteoblast formation and promoting osteoclast differentiation through the upregulation of NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Ouyang,Shimin Li,Yunzhi Ding,Feng Xin,Meng LiuPubMed 35642703
- Journal of proteome research 21:654-6702022NMR Metabolomics Assessment of Osteogenic Differentiation of Adipose-Tissue-Derived Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela S C Bispo,Catarina S H Jesus,Marlene Correia,Filipa Ferreira,Giulia Bonifazio,Brian J Goodfellow,Mariana B Oliveira,João F Mano,Ana M GilPubMed 35061379
- BioMed research international 2021:70314922021Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton's Jelly-Derived Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsghar Fallah,Mahdieh Alipour,Zahra Jamali,Akbar Farjadfar,Leila Roshangar,Minoo Partovi Nasr,Parisa Hashemi,Marziyeh AghazadehPubMed 34790821
- Folia histochemica et cytobiologica 59:187-1942021SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Xiong,Xin Guo,Xianhua CaiPubMed 34580847
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 52.08 - 3333 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13.99 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation (PubMed:3019668, PubMed:6967872). Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium (PubMed:6967872). The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.
Alternative names
Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP, BGLAP
What's included?
Recommended products
Human Osteocalcin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteocalcin with a sensitivity of 13.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 52.08 - 3333 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 13.99 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 80 - 103 % |
Sample type EDTA Plasma | Average % = 93 | Range 90 - 96 % |
Sample type Cell culture media | Average % = 102 | Range 91 - 115 % |
Sample type Heparin Plasma | Average % = 93 | Range 81 - 99 % |
Sample type Citrate plasma | Average % = 90 | Range 84 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Osteocalcin ELISA Kit (ab270202) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteocalcin protein in cell culture media, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Osteocalcin with 13.99 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Osteocalcin is a small secreted protein 49 amino acids produced by osteoblasts. Osteocalcin acts as a hormone impacting bone mineral absorption, glucose metabolism and insulin secretion. During bone reabsorption osteoclasts break down the bone matrix releasing bound Osteocalcin into 5 different fragments; N-terminal, Mid, C-terminal, N-terminal-Mid, and Mid-C-terminal into the serum. Circulating levels of intact Osteocalcin and Osteocalcin fragments have been found to vary significantly between healthy individuals and diseased stats such as Paget’s disease, osteoporosis, fracture, and renal failure. The standard protein in this product is mature Osteocalcin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.
- Biological function summary
Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.
- Pathways
Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).
- Associated diseases and disorders
Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)
Human Osteocalcin competitor curve comparison.
Standard Curve comparison between human osteocalcin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)
Human osteocalcin standard curve comparison.
Standard Curve comparison between the original Human Ostecalcin SimpleStep ELISA kit (ab270202) and current Human Ostecalcin SimpleStep ELISA kit (ab195214).
Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)
Example of human Osteocalcin standard curve in Sample Diluent NS.
The Osteocalcin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)
Interpolated concentrations of native Osteocalcin in human serum and plasma samples.
The concentrations of Osteocalcin were measured in duplicates, interpolated from the Osteocalcin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 10%, plasma (heparin) 7.5%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 34.49 ng/mL in serum, 24.94 ng/mL in plasma (citrate), 41.51 ng/ml in plasma (heparin), and 32.69 ng/ml in plasma (EDTA).
Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 18.95 ng/mL with a range of 1.30 – 38.94 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com