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AB313916

Human Osteocrin ELISA Kit

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Human Osteocrin ELISA Kit is a Sandwich ELISA for the measurement of Human Osteocrin in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Osteocrin, Musclin, OSTN

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Sandwich ELISA - Human Osteocrin ELISA Kit (AB313916)
  • sELISA

Supplier Data

Sandwich ELISA - Human Osteocrin ELISA Kit (AB313916)

Example of standard curve using ab313916. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.55 ng/mL

Range

0.55 - 125 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB63301

Recombinant Human Osteocrin protein

SDS-PAGE - Recombinant Human Osteocrin protein (AB63301)

  • 0
  • 0
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Osteocrin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Osteocrin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Osteocrin coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "81 - 145 %", "average": "= 120.1" }, { "sample": "Plasma", "range": "102 - 137 %", "average": "= 125.1" }, { "sample": "Cell culture media", "range": "122 - 143 %", "average": "= 135" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313916-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313916-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313916-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313916-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Osteocrin Detection Antibody", "number":"AB313916-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313916-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313916-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313916-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Osteocrin Protein Standard", "number":"AB313916-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Osteocrin Antibody-coated ELISA Plate", "number":"AB313916-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313916-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteocrin also known as OSTN is a small secreted protein with an approximate molecular mass of 13 kDa. It is expressed highly in certain tissues such as bone and muscle. Osteocrin acts mechanically by binding to natriuretic peptide receptor-C (NPR-C). This interaction plays a substantial role in mediating various physiological responses.
Biological function summary

Osteocrin participates in the modulation of bone growth and muscle activity. It exists as part of a non-complex pathway serving as a regulatory molecule for natriuretic peptides. Osteocrin modulates the effects of natriuretic peptides by preventing their binding to clearance receptors which increases their bioavailability and prolonged action. This action impacts vascular physiology and growth regulation.

Pathways

Osteocrin plays a significant role in the natriuretic peptide signaling pathway. In this pathway it functions closely with proteins such as ANP (atrial natriuretic peptide) and BNP (brain natriuretic peptide). These proteins engage in blood pressure and volume regulation by causing vasodilation and promoting natriuresis. Osteocrin modulates the effectiveness of this pathway through its interaction with NPR-C enhancing the functions by extending the half-life of the active peptides involved.

Researchers have identified osteocrin's involvement in cardiovascular conditions and osteoporosis. Osteocrin's ability to enhance natriuretic peptide availability has implications for managing cardiovascular diseases where these peptides play protective roles. Additionally osteocrin influences bone density suggesting a connection to osteoporosis. Interactions with proteins like NPR-C and BNP are critical in these conditions with osteocrin modulating their biological activity and potentially offering therapeutic benefits.
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Product protocols

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Target data

Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience (PubMed : 27830782). Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex (PubMed : 27830782). Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production (By similarity).
See full target information Osteocrin
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websiteProtocolBooklet
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Product promise

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