Human Osteopontin ELISA Kit

• ELISA

Supplier Data

ELISA - Human Osteopontin ELISA Kit (AB269374)

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 19 ng/mL with a range of 10 – 39 ng/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Interpolated concentrations of native Osteopontin in human milk samples.

The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 88 μg/mL in milk.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Interpolated concentrations of human Osteopontin in CSF.

Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : CSF 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Interpolated concentrations of native Osteopontin in human urine and stimulated PBMC cell culture supernatant samples.

The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 400 and stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 1,516 ng/mL in urine and 4.2 ng/mL in stimulated PBMC cell culture supernatant. No detectable signal was observed in unstimulated PBMC cell culture supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Human Osteopontin standard curve comparison.

Standard Curve comparison between human Osteopontin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Example of human Osteopontin standard curve in Sample Diluent NS.

The Osteopontin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit (AB269374)

Interpolated concentrations of native Osteopontin in human serum and plasma samples.

The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 16.2 ng/mL in serum, 13.5 ng/mL in plasma (citrate), 13.0 ng/mL in plasma (EDTA), and 19.9 ng/mL in plasma (heparin).