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AB326001

Human Osteopontin ELISA Kit, Chemiluminescent

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Human Osteopontin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Osteopontin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 28.81 - 63000 pg/ml with a sensitivity of 6.32 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1

8 Images
Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Interpolated concentrations of human Osteopontin in CSF. Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : CSF 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Interpolated concentrations of native Osteopontin in human serum and plasma samples. The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 16.2 ng/mL in serum, 13.5 ng/mL in plasma (citrate), 13.0 ng/mL in plasma (EDTA), and 19.9 ng/mL in plasma (heparin).

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Dynamic range comparison between the Chemiluminescent Human Osteopontin SimpleStep ELISA, colorimetric Human Osteopontin SimpleStep ELISA (ab269374), and leading colorimetric/chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Standard curve comparison between the Chemiluminescent Human Osteopontin SimpleStep ELISA and colorimetric Human Osteopontin SimpleStep ELISA (ab269374) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Lab

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Interpolated concentrations of native Osteopontin in human urine and stimulated PBMC cell culture supernatant samples. The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 400 and stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 1,516 ng/mL in urine and 4.2 ng/mL in stimulated PBMC cell culture supernatant. No detectable signal was observed in unstimulated PBMC cell culture supernatant.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 19 ng/mL with a range of 10 – 39 ng/mL.

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)
  • sELISA

Supplier Data

Sandwich ELISA - Human Osteopontin ELISA Kit, Chemiluminescent (AB326001)

Interpolated concentrations of native Osteopontin in human milk samples. The concentrations of Osteopontin were measured in duplicates, interpolated from the Osteopontin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteopontin concentration was determined to be 88 μg/mL in milk.

Key facts

Detection method

Luminescent

Sample types

Cerebral Spinal Fluid, Cell culture supernatant, Serum, Milk, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.32 pg/mL

Range

28.81 - 63000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Osteopontin ELISA Kit, Chemiluminescent (ab326001) is a rapid single-wash sandwich 55-min ELISA to measure Human Osteopontin in Cerebral Spinal Fluid, Cell culture supernatant, Serum, Milk, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma with a sensitivity of 6.32 pg/mL.

How the assay works

Human Osteopontin ELISA Kit, Chemiluminescent (ab326001) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Osteopontin (ab326001) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Osteopontin antibody [EPR21139-218] - BSA and Azide free (Capture) ab269410
- Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (Detector) ab269411
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "82 - 93 %", "average": "= 86" }, { "sample": "Cell culture supernatant", "range": "102 - 115 %", "average": "= 108" }, { "sample": "Serum", "range": "99 - 104 %", "average": "= 102" }, { "sample": "Milk", "range": "93 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "95 - 102 %", "average": "= 99" }, { "sample": "Urine", "range": "96 - 100 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "91 - 93 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information SPP1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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