Human Osteoprotegerin ELISA Kit is a sandwich ELISA designed to quantify Human Osteoprotegerin with a sensitivity of 1 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.23 - 900 pg/mL
- Cited in over 10 publications
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- BMC oral health 24:522024Postmenopausal women with HIV have increased tooth loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunil Wadhwa,Taylor R Finn,Karolina Kister,Satoko Matsumura,Michael Levit,Anyelina Cantos,Jayesh Shah,Bruno Bohn,Evanthia Lalla,John T Grbic,Ryan T Demmer,Michael T YinPubMed 38191383
- British journal of pharmacology 181:429-4462023Neutrophil extracellular traps mediate bone erosion in rheumatoid arthritis by enhancing RANKL-induced osteoclastogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyda Henriques Schneider,Thaise Mayumi Taira,Gabriel Azevedo Públio,Douglas da Silva Prado,Paula Barbim Donate Yabuta,Jéssica Cristina Dos Santos,Caio Cavalcante Machado,Flávio Falcão Lima de Souza,Lucas Gabriel Rodrigues Venturini,Renê Donizeti Ribeiro de Oliveira,Thiago Mattar Cunha,José Carlos Alves-Filho,Paulo Louzada-Júnior,Tarcília Aparecida da Silva,Sandra Yasuyo Fukada,Fernando Queiróz CunhaPubMed 37625900
- Materials (Basel, Switzerland) 15:2022Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Fernández-Lizárraga,Julieta García-López,Sandra E Rodil,Rosa María Ribas-Aparicio,Phaedra Silva-BermudezPubMed 35955174
- The Korean journal of internal medicine 37:821-8292022Benefits of lumican on human bone health: clinical evidence using bone marrow aspirates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Sun Lee,So Jeong Park,Jin Young Lee,Eunah Choi,Beom-Jun KimPubMed 35468703
- Polymers 13:2021Synergies of Human Umbilical Vein Endothelial Cell-Laden Calcium Silicate-Activated Gelatin Methacrylate for Accelerating 3D Human Dental Pulp Stem Cell Differentiation for Endodontic Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Yun Lai,Tzu-Hsin Lee,Jian-Xun Chen,Hooi-Yee Ng,Tsui-Hsien Huang,Ming-You ShiePubMed 34641117
- Acta biomaterialia 135:689-7042021Biomimetic versus sintered macroporous calcium phosphate scaffolds enhanced bone regeneration and human mesenchymal stromal cell engraftment in calvarial defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeadhbh Á Brennan,David S Monahan,Bénédicte Brulin,Sara Gallinetti,Paul Humbert,Christina Tringides,Cristina Canal,Maria Pau Ginebra,Pierre LayrollePubMed 34520883
- Diagnostics (Basel, Switzerland) 11:2021A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdoardo Gaude,Barbara Nogueira,Marcos Ladreda Mochales,Sheila Graham,Sarah Smith,Lisa Shaw,Sara Graziadio,Gonzalo Ladreda Mochales,Philip Sloan,Joshua D Bernstock,Shashank Shekhar,Toby I Gropen,Christopher I PricePubMed 34206615
- CJC open 3:470-4782021Serum TRACP5b, a Marker of Bone Resorption, Is Associated With Adverse Cardiac Prognosis in Hospitalized Patients With Heart Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Abe,Akiomi Yoshihisa,Yasuhiro Ichijo,Yusuke Kimishima,Tetsuro Yokokawa,Tomofumi Misaka,Takamasa Sato,Masayoshi Oikawa,Atsushi Kobayashi,Takashi Kaneshiro,Kazuhiko Nakazato,Yasuchika TakeishiPubMed 34027350
- Journal of infection in developing countries 15:289-2962021Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSakaewan Ounjaijean,Kanokwan Kulprachakarn,Linda Aurpibul,Quanhathai Kaewpoowat,Kongsak Boonyapranai,Romanee Chaiwarith,Supapong Arwon,Khuanchai Supparatpinyo,Kittipan RerkasemPubMed 33690213
- International forum of allergy & rhinology 10:364-3732019Receptor activator of nuclear factor κB ligand is a biomarker for osteitis of chronic rhinosinusitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIl Gyu Kong,Dong-Kyu Kim,Kyoung Mi Eun,Seung Koo Yang,Minju Kim,Heonjeong Oh,Dae Woo KimPubMed 31693309
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.23 - 900 pg/mL
- Sensitivity
- < 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Alternative names
OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin
What's included?
Recommended products
Human Osteoprotegerin ELISA Kit is a sandwich ELISA designed to quantify Human Osteoprotegerin with a sensitivity of 1 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.23 - 900 pg/mL
- Cited in over 10 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.23 - 900 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 1 pg/mL
- General recovery
- 100%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99.75 | Range 89 - 108 % |
Sample type Serum | Average % = 99.85 | Range 89 - 108 % |
Sample type Plasma | Average % = 102.45 | Range 90 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Osteoprotegerin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoprotegerin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Osteoprotegerin ELISA Kit (Human Osteoprotegerin ELISA Kit ab189580) from our SimpleStep ELISA® range.
Produced using a non-baculovirus expression system.
Optimization may be required with urine samples.
We recommend preparing serum-free or low-serum medium samples, as serum tends to contain cytokines which may produce significant background signals. If it is necessary to test serum-containing medium, we recommend also running an uncultured media blank to assess baseline signals. This baseline can then be subtracted from the cultured media sample data.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
- Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
- Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
- Associated diseases and disorders
OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
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4 product images
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab100617)
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab100617)
Osteoprotegerin measured in cell lysates showing quantity (pg) per mg of tested sample
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab100617)
Representative Standard Curve using ab100617.
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab100617)
Representative Standard Curve using ab100617.
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