Human Osteoprotegerin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteoprotegerin with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- PloS one 17:e02763282022Xianling Gubao attenuates high glucose-induced bone metabolism disorder in MG63 osteoblast-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinlong Chen,Yan Li,Zhongwen Zhang,Liping Chen,Yaqian Liu,Shuhong Huang,Xiaoqian ZhangPubMed 36548302
- JBMR plus 6:e105972022Open-Label Pilot Study of Interferon Gamma-1b in Patients With Non-Infantile Osteopetrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew Nguyen,Weston P Miller,Ashish Gupta,Troy C Lund,Daniel Schiferl,Lok Sze Kelvin Lam,Zorayr Arzumanyan,Paul J Orchard,Lynda E PolgreenPubMed 35309862
- Circulation journal : official journal of the Japanese Circulation Society 85:69-762020Association of the Serum Osteoprotegerin Level With Target Organ Damage in Patients at High Risk of Coronary Artery Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaehoon Chung,Hack-Lyoung Kim,Jung Pyo Lee,Woo-Hyun Lim,Jae-Bin Seo,Sang-Hyun Kim,Joo-Hee Zo,Myung-A KimPubMed 33250498
- International journal of molecular sciences 19:2018Influence of Implant Material and Surface on Differentiation and Proliferation of Human Adipose-Derived Stromal Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Jung et. al.PubMed 30551618
- Osteoarthritis and cartilage 26:797-8062018Mutations in osteoprotegerin account for the CCAL1 locus in calcium pyrophosphate deposition disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC J Williams,U Qazi,M Bernstein,A Charniak,C Gohr,E Mitton-Fitzgerald,A Ortiz,L Cardinal,A T Kaell,A K RosenthalPubMed 29578045
- Acta biomaterialia 42:378-3882016Osteoprotegerin gene-modified BMSCs with hydroxyapatite scaffold for treating critical-sized mandibular defects in ovariectomized osteoporotic rats.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 11 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Alternative names
OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin
What's included?
Recommended products
Human Osteoprotegerin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteoprotegerin with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 11 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101 | Range 100 - 103 % |
Sample type EDTA Plasma | Average % = 98 | Range 86 - 104 % |
Sample type Heparin Plasma | Average % = 89 | Range 84 - 92 % |
Sample type Citrate plasma | Average % = 88 | Range 86 - 90 % |
Sample type Cell culture media | Average % = 88 | Range 81 - 96 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Osteoprotegerin ELISA Kit (ab189580) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Osteoprotegerin with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Osteoprotegerin (OPG), also known as Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a 46kDa glycoprotein member of the tumor necrosis factor receptor superfamily. This cytokine is a homodimer expressed and secreted by multiple adult tissues such as the lung, liver, spleen, prostate, ovary, adrenal gland and bone marrow. It plays a critical function in bone homeostasis by acting as a decoy receptor for TNFSF11/RANKL and for the receptor activator of nuclear factor-kappa-B ligand. OPG therefore decreases bone resorption by inhibiting osteoclast differentiation and activation of osteoclast and by stimulating osteoclast apoptosis.
OPG deficiency has been found in Juvenile Paget disease, a rare autosomal recessive osteopathy that presents in infancy and early childhood characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures and deformities due to accelerated bone remodeling. OPG may also play a role in arterial calcification and high levels of OPG have been found to be an independent risk factor for cardiovascular disease and vascular mortality.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
- Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
- Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
- Associated diseases and disorders
OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
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3 product images
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab189580)
Example of Osteoprotegerin standard curve in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab189580)
Example of Osteoprotegerin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Osteoprotegerin ELISA Kit (ab189580)
Ten individual healthy donors were evaluated for the presence of Osteoprotegerin in serum using this assay.
Results were interpolated from the standard curve in Sample Diluent 25BS and corrected by sample dilution (1:2). The mean level of Osteoprotegerin is 378.8 pg/mL with a range of 256.5 – 584.5 pg/mL and a standard deviation of 106.7 pg/mL.
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