Human Osteoprotegerin ELISA Kit

Product details

Human Osteoprotegerin ELISA Kit (ab189580) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Osteoprotegerin with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Osteoprotegerin (OPG), also known as Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a 46kDa glycoprotein member of the tumor necrosis factor receptor superfamily. This cytokine is a homodimer expressed and secreted by multiple adult tissues such as the lung, liver, spleen, prostate, ovary, adrenal gland and bone marrow. It plays a critical function in bone homeostasis by acting as a decoy receptor for TNFSF11/RANKL and for the receptor activator of nuclear factor-kappa-B ligand. OPG therefore decreases bone resorption by inhibiting osteoclast differentiation and activation of osteoclast and by stimulating osteoclast apoptosis.

OPG deficiency has been found in Juvenile Paget disease, a rare autosomal recessive osteopathy that presents in infancy and early childhood characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures and deformities due to accelerated bone remodeling. OPG may also play a role in arterial calcification and high levels of OPG have been found to be an independent risk factor for cardiovascular disease and vascular mortality.