Human OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human OX40 (TNFRSF4) in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
46.9 - 3000 pg/mL
1h 30m
= 23 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.
TXGP1L, TNFRSF4, TXGP1L, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor
Human OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human OX40 (TNFRSF4) in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
46.9 - 3000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 23 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Cell Media | n 5 | mean - | SD - | C.V. 3.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Cell Media | n 3 | mean - | SD - | C.V. 4.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 120 | Range 116 - 122 % |
Sample type EDTA Plasma | Average % = 115 | Range 112 - 117 % |
Sample type Cell culture extracts | Average % = 106 | Range 102 - 111 % |
Sample type Heparin Plasma | Average % = 120 | Range 116 - 121 % |
Sample type Citrate plasma | Average % = 116 | Range 113 - 117 % |
Sample type Cell culture media | Average % = 113 | Range 112 - 114 % |
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Human OX40 ELISA Kit (TNFRSF4) (ab231926) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cit plasma, edta plasma, hep plasma, serum, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OX40 with 23 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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This supplementary information is collated from multiple sources and compiled automatically.
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
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Example of human OX40 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Example of human OX40 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of spiked OX40 in human serum and plasma samples.
The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of OX40 were measured.
Interpolated concentrations of native OX40 in human cell culture supernatant sample.
The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 296 pg/mL in Stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.
Interpolated concentrations of native OX40 in human Jurkat Cell Extract, sample based on a 4,000 μg/mL extract load.
The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 419 pg/mL in Jurkat Cell Extract.
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