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Human OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human OX40 (TNFRSF4) in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926), expandable thumbnail
  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926), expandable thumbnail
  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926), expandable thumbnail
  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926), expandable thumbnail
  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Sensitivity

= 23 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human OX40 Capture Antibody
1 x 600 µL
10X Human OX40 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BI
1 x 6 mL
Human OX40 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human OX40 (TNFRSF4) in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 23 pg/mL

Precision

Intra assay

Sample

Cell Media

n

5

mean

-

SD

-

C.V.

3.6

Inter assay

Sample

Cell Media

n

3

mean

-

SD

-

C.V.

4.9

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 120

Range

116 - 122 %

Sample type

EDTA Plasma

Average %

= 115

Range

112 - 117 %

Sample type

Cell culture extracts

Average %

= 106

Range

102 - 111 %

Sample type

Heparin Plasma

Average %

= 120

Range

116 - 121 %

Sample type

Citrate plasma

Average %

= 116

Range

113 - 117 %

Sample type

Cell culture media

Average %

= 113

Range

112 - 114 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human OX40 ELISA Kit (TNFRSF4) (ab231926) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cit plasma, edta plasma, hep plasma, serum, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OX40 with 23 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

Associated diseases and disorders

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926), expandable thumbnail

    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)

    Example of human OX40 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926), expandable thumbnail

    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)

    Example of human OX40 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926), expandable thumbnail

    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)

    Interpolated concentrations of spiked OX40 in human serum and plasma samples.

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of OX40 were measured.

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926), expandable thumbnail

    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)

    Interpolated concentrations of native OX40 in human cell culture supernatant sample.

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 296 pg/mL in Stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926), expandable thumbnail

    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)

    Interpolated concentrations of native OX40 in human Jurkat Cell Extract, sample based on a 4,000 μg/mL extract load.

    The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 419 pg/mL in Jurkat Cell Extract.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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