AB285269
Human OxLDL ELISA Kit is a sandwich ELISA designed to quantify Human OxLDL with a sensitivity of 1.875 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 3.125 - 200 ng/mL
View Alternative Names
CLEC8A, LOX1, OLR1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human OxLDL ELISA Kit (AB285269)
Human OxLDL ELISA Kit.
Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
Human OxLDL ELISA Kit ab285269 is a sandwich ELISA to measure Human OxLDL in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids with a sensitivity of 1.875 ng/ml.
How the assay works
The density of color is proportional to the amount of OxLDL captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human OxLDL ELISA Kit ab285269 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
How the assay works
The density of color is proportional to the amount of OxLDL captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human OxLDL ELISA Kit ab285269 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.. (Microbial infection) May serve as a receptor for adhesin A variant 3 (nadA) of N.meningitidis.
Product promise
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