Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Oxytocin-neurophysin 1 Prepropeptide with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PubMed:18174156).
Alternative names
OT, OXT, Oxytocin-neurophysin 1, OT-NPI
What's included?
Recommended products
Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Oxytocin-neurophysin 1 Prepropeptide with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 10 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 84 - 100 % |
Sample type EDTA Plasma | Average % = 104 | Range 95 - 111 % |
Sample type Heparin Plasma | Average % = 104 | Range 94 - 113 % |
Sample type Citrate plasma | Average % = 92 | Range 83 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit ab242248 is a rapid single-wash 90-min Sandwich ELISA to measure Human Oxytocin-neurophysin 1 Prepropeptide in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10 pg/mL.
How the assay works
Human Oxytocin-neurophysin 1 Prepropeptide SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit ab242248 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oxytocin-neurophysin 1 also known as prepro-oxytocin-neurophysin I is a precursor protein with a molecular weight of approximately 20 kDa. This protein is expressed primarily in the hypothalamus and is involved in the synthesis and storage of the hormone oxytocin a peptide essential for various physiological processes. During synthesis the protein undergoes cleavage to separate oxytocin and neurophysin 1 a carrier protein that aids in the transport and stabilization of oxytocin in neurosecretory granules.
- Biological function summary
Oxytocin-neurophysin 1 plays an important role in the regulation of social behaviors reproductive functions and fluid homeostasis. This precursor is not part of a larger protein complex but neurophysin 1 serves as an essential carrier that binds and transports oxytocin to its target sites in the body. Oxytocin itself functions as a neuromodulator and hormone exerting effects on the uterine muscles and mammary glands promoting childbirth and lactation.
- Pathways
The biosynthesis and release of oxytocin-neurophysin 1 are closely associated with the hypothalamic neurosecretory pathways. These pathways involve the neurohypophysis where oxytocin is released into the bloodstream. Additionally oxytocin interacts with the G-protein-coupled receptor OXTR to elicit its physiological effects. V1a and V2 receptors alongside oxytocin play roles in various signaling pathways that affect metabolic and cardiovascular functions.
- Associated diseases and disorders
Dysregulation of oxytocin-neurophysin 1 has been linked to the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and autism spectrum disorders. In these conditions altered levels of oxytocin affect fluid balance and social behavior. Additionally changes in oxytocin levels often in tandem with altered vasopressin regulation associate with the development and exacerbation of these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (ab242248)
Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (ab242248)
Example of human Oxytocin-neurophysin 1 standard curve in Sample Diluent NS.
The Oxytocin-neurophysin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (ab242248)
Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum and plasma samples.
The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 23,301 pg/mL in serum, 13,420 pg/mL in plasma (citrate), 21,214 pg/mL in plasma (EDTA), and 20843 pg/mL in plasma (Heparin).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com