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AB100621

Human P cadherin ELISA Kit

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Human P cadherin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human P cadherin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin

2 Images
Sandwich ELISA - Human P cadherin ELISA Kit (AB100621)
  • sELISA

Supplier Data

Sandwich ELISA - Human P cadherin ELISA Kit (AB100621)

Representative Standard Curve using ab100621.

Sandwich ELISA - Human P cadherin ELISA Kit (AB100621)
  • sELISA

Supplier Data

Sandwich ELISA - Human P cadherin ELISA Kit (AB100621)

Representative Standard Curve using ab100621.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

2.47 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's P cadherin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human P cadherin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human P cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and P cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human P cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "117 - 138 %", "average": "= 129.1" }, { "sample": "Serum", "range": "111 - 131 %", "average": "= 121.3" }, { "sample": "Plasma", "range": "105 - 125 %", "average": "= 114.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100621-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100621-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100621-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"P cadherin Microplate (12 x 8 wells)", "number":"AB100621-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100621-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100621-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human P cadherin Standard (lyophilized)", "number":"AB100621-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human P cadherin", "number":"AB100621-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100621-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.
Biological function summary

P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.

Pathways

Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.

P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.
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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
See full target information CDH3
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websiteProtocolBooklet
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Product promise

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