AB274395

Human P-cadherin ELISA Kit (CDH3)

Recombinant
SimpleStep
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Human P-cadherin ELISA Kit (CDH3) is a single-wash 90-min Simplestep used to quantify Human P-cadherin (CDH3) with a sensitivity of 77.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin

3 Images

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

sELISA

Supplier Data

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Example of human P-cadherin standard curve in Sample Diluent 25BS.

The P-cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Interpolated concentrations of native P-cadherin in human serum and plasma samples.

The concentrations of P-cadherin were measured in duplicates, interpolated from the P-cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean P-cadherin concentration was determined to be 9,438 pg/mL in serum, 7,171 pg/mL in plasma (heparin), and 5,427 pg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean P-cadherin concentration was determined to be 7,429 pg/mL with a range of 4,127 – 9,721 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 77.07 pg/mL

Range

234.38 - 15000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human P-cadherin ELISA kit (ab274395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human P-caderin protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human P-cadherin with 77.07 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

P-cadherin (CDH3) is a member of the cadherin family of calcium-dependent adhesion proteins. P-cadherin is expressed in epithelial cells and some cancers. Soluble P-cadherin expression is correlated with tumor stage in non-invasive lung cancer and is a target for potential therapeutic treatment of cancer.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]

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Recovery

[ { "sample": "Serum", "range": "101 - 123 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "93 - 101 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "89 - 97 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.

Biological function summary

P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.

Pathways

Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.

P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.

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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH3

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human P-cadherin ELISA Kit (CDH3)

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Sandwich ELISA - Human P-cadherin ELISA Kit (CDH3) (AB274395)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise