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AB273160

Human P-Selectin (CD62P) ELISA kit

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Human P-Selectin (CD62P) ELISA kit is a Sandwich ELISA for the measurement of Human P-Selectin (CD62P) in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

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ELISA - Human P-Selectin (CD62P) ELISA kit (AB273160)
  • ELISA

Supplier Data

ELISA - Human P-Selectin (CD62P) ELISA kit (AB273160)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example of Human P-selectin ELISA kit (CD62P) standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Other biological fluids, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.6 ng/mL

Range

4.4 - 140 ng/mL

Assay time

2h 45m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human P-selectin ELISA kit (CD62P) (ab273160) is for the in-vitro qualitative and quantitative determination of CD62P in supernatants, buffered solutions or serum and plasma samples. This assay will recognize both natural and recombinant human CD62P.

A capture antibody highly specific for CD62P has been coated to the wells of the microtiter strip plate. Binding of CD62P in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.

During the next incubation period the binding of the biotinylated anti-CD62P secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.

The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.

A chromogen substrate is added to the wells resulting in the progressive development of a blue colored complex with the conjugate. The color development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced colored complex is directly proportional to the concentration of CD62P present in the samples and standards.

The absorbance of the color complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD62P in any sample tested.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 18, "mean": "92 ng/mL", "standardDeviation": "6.9", "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 18, "mean": "42.3 ng/mL", "standardDeviation": "1.8", "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 6, "mean": "90.4 ng/mL", "standardDeviation": "5", "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 6, "mean": "39.6 ng/mL", "standardDeviation": "3.1", "coefficientOfVariability": "7.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273160-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB273160-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD62P Standard", "number":"AB273160-CMP11", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Cover", "number":"AB273160-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody diluent", "number":"AB273160-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Wash Buffer", "number":"AB273160-CMP06", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated Antibody CD62P", "number":"AB273160-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"TMB Substrate", "number":"AB273160-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Standard diluent Buffer", "number":"AB273160-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB273160-CMP08", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB273160-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD62P Coated Microwell strips", "number":"AB273160-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62P also known as P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It is a type of protein with a known molecular weight of approximately 140 kDa. P-selectin plays a mechanical role in mediating leukocyte rolling on the endothelium which is an initial step in the inflammatory response. This protein is rapidly translocated to the plasma membrane upon platelet activation and is stored in alpha granules of platelets and Weibel-Palade bodies of endothelial cells.
Biological function summary

P-selectin is critical in the recruitment of leukocytes to sites of tissue injury and infection. It facilitates the adhesion of leukocytes to the vascular endothelium by binding to its ligand PSGL-1 on the leukocyte surface. This interaction supports the transition of leukocytes from the circulation to the site of inflammation. P-selectin is also involved in thrombus formation as it contributes to the interactions between platelets leukocytes and endothelium in the vascular wall. It acts as part of a dynamic complex in the regulation of the innate immune response and hemostasis.

Pathways

CD62P plays an important role in both the inflammatory and coagulation pathways. It is an important mediator in the leukocyte adhesion cascade influencing leukocyte tethering and rolling on activated endothelium. The interaction of P-selectin with PSGL-1 initiates the signaling pathways that lead to firm adhesion and extravasation of leukocytes. In the coagulation pathway P-selectin enhances interactions between platelets and leukocytes which support thrombus formation. Its regulation and activity are tightly linked with other proteins such as E-selectin and L-selectin in coordinating vascular and systemic inflammatory responses.

Altered expression of CD62P is associated with various inflammatory conditions and cardiovascular diseases. In thrombosis increased levels of P-selectin enhance platelet aggregation and stability of the thrombus contributing to pathological clot formation. Similarly in atherosclerosis P-selectin facilitates the recruitment of monocytes to the vessel wall promoting plaque development. The protein's function relates to interactions with key inflammatory mediators and its dysregulation is often observed in conditions like chronic inflammatory diseases and acute myocardial infarction.
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Product protocols

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Target data

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (PubMed : 37184585).
See full target information SELP
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websiteProtocolBooklet
en
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