Human p21 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human p21 in Tissue Lysate, Cell culture extracts samples.
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- Biotechnology and applied biochemistry 70:1597-16152023Effective-by-method for the preparation of folic acid-coated TiO nanoparticles with high targeting potential for apoptosis induction against bladder cancer cells (T24).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDemiana H Hanna,Marina M Aziz,E El ShafeePubMed 36905187
- Pharmacological reports : PR 72:214-2242020Effect of new olivacine derivatives on p53 protein level.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Gębarowski et. al.PubMed 32016852
- Future medicinal chemistry 10:2771-27892018One-pot synthesis of spiro(indoline-3,4'-pyrazolo[3,4-b]pyridine)-5'-carbonitriles as p53-MDM2 interaction inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHany S Ibrahim et. al.PubMed 30526032
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell culture extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 11 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657).
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, CDKN1A, MDA6, CAP20, CDKN1, CIP1, PIC1, SDI1, WAF1
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Human p21 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human p21 in Tissue Lysate, Cell culture extracts samples.
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, CDKN1A, MDA6, CAP20, CDKN1, CIP1, PIC1, SDI1, WAF1
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell culture extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate
- Sensitivity
= 11 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample MCF7 Lysate | n 5 | mean - | SD - | C.V. 4.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample MCF7 Lysate | n 3 | mean - | SD - | C.V. 7.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 120 | Range 115 - 129 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human p21 ELISA Kit (ab214658) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p21 protein in tissue lysate and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human p21 with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
p21 plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. Upregulation of p21 mRNA and protein following ionizing radiation is dependent on p53 (TP53), and p21 mediates cell cycle arrest in response to the p53 checkpoint pathway. p21 binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. p21 functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, p21 inhibits the kinase activity of the cyclin D-CDK4 complex.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.
- Biological function summary
P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.
- Pathways
P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.
- Associated diseases and disorders
P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.
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3 product images
ELISA - Human p21 ELISA Kit (ab214658)
Example of human p21 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human p21 ELISA Kit (ab214658)
Interpolated concentration of native p21 in human extract samples of MCF-7 cells treated for 18 hours with 1 M camptothecin based on 8 μg/mL extract load.
The concentrations of p21 were measured in duplicates in a two-fold dilution series and interpolated from the p21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p21 concentration was determined to be 1,811 pg/mL in extract sample of MCF-7 cells treated for 18 hours with 1 mM camptothecin.
ELISA - Human p21 ELISA Kit (ab214658)
Interpolated concentrations of native p21 in human extract samples of HeLa cells, vehicle (DMSO)-treated MCF-7 cells and 18 hours 1 μM camptothecin-treated MCF-7 cells samples. The concentrations of p21 were measured in duplicates at 3 different dilutions, interpolated from the p21 standard curve. Note that Undiluted HeLa cell extract and Undiluted vehicle (DMSO)-treated MCF-7cell extract samples were at 32 g/mL. Note that Undiluted 18 hours 1 M camptothecin-treated MCF-7 cells samples were at 8 μg/mL. The interpolated, dilution factor-corrected values are graphed in pg of p21 per μg of total protein (mean +/- SD, n=2).
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