AB214658

Human p21 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human p21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human p21 with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, CDKN1A, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6

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ELISA

Supplier Data

ELISA - Human p21 ELISA Kit (AB214658)

ELISA

Unknown

ELISA - Human p21 ELISA Kit (AB214658)

Interpolated concentrations of native p21 in human extract samples of HeLa cells, vehicle (DMSO)-treated MCF-7 cells and 18 hours 1 μM camptothecin-treated MCF-7 cells samples. The concentrations of p21 were measured in duplicates at 3 different dilutions, interpolated from the p21 standard curve. Note that Undiluted HeLa cell extract and Undiluted vehicle (DMSO)-treated MCF-7cell extract samples were at 32 µg/mL. Note that Undiluted 18 hours 1 µM camptothecin-treated MCF-7 cells samples were at 8 μg/mL. The interpolated, dilution factor-corrected values are graphed in pg of p21 per μg of total protein (mean +/- SD, n=2).

ELISA

Unknown

ELISA - Human p21 ELISA Kit (AB214658)

Interpolated concentration of native p21 in human extract samples of MCF-7 cells treated for 18 hours with 1 µM camptothecin based on 8 μg/mL extract load.

The concentrations of p21 were measured in duplicates in a two-fold dilution series and interpolated from the p21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p21 concentration was determined to be 1,811 pg/mL in extract sample of MCF-7 cells treated for 18 hours with 1 mM camptothecin.

ELISA

Unknown

ELISA - Human p21 ELISA Kit (AB214658)

Example of human p21 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human p21 ELISA Kit ab214658 is a rapid single-wash 90-min Sandwich ELISA to measure Human p21 in cell culture extracts, tissue lysate. This SimpleStep sensitivity is 11 pg/mL.

How the assay works

Human p21 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human p21 ELISA Kit ab214658 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-p21 antibody [EPR18690-121] - BSA and Azide free (Capture) ab242711
- Anti-p21 antibody [EPR18690-103] - BSA and Azide free (Detector) ab242711

p21 plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. Upregulation of p21 mRNA and protein following ionizing radiation is dependent on p53 (TP53), and p21 mediates cell cycle arrest in response to the p53 checkpoint pathway. p21 binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. p21 functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, p21 inhibits the kinase activity of the cyclin D-CDK4 complex.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "MCF7 Lysate", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "MCF7 Lysate", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "115 - 129 %", "average": "= 120" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214658-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214658-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214658-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214658-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214658-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214658-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214658-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB214658-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human p21 Capture Antibody", "number":"AB214658-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human p21 Lyophilized Recombinant Protein", "number":"AB214658-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human p21 Detector Antibody", "number":"AB214658-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214658-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214658-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.

Biological function summary

P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.

Pathways

P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-?) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.

P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.

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Product protocols

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Target data

Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed : 9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed : 11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity).

See full target information CDKN1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology and applied biochemistry 70:1597-1615 PubMed36905187

2023

Effective-by-method for the preparation of folic acid-coated TiO nanoparticles with high targeting potential for apoptosis induction against bladder cancer cells (T24).

Applications

Unspecified application

Species

Unspecified reactive species

Demiana H Hanna,Marina M Aziz,E El Shafee

Pharmacological reports : PR 72:214-224 PubMed32016852

2020

Effect of new olivacine derivatives on p53 protein level.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Gębarowski,Benita Wiatrak,Katarzyna Gębczak,Beata Tylińska,Kazimierz Gąsiorowski

Future medicinal chemistry 10:2771-2789 PubMed30526032

2018

One-pot synthesis of spiro(indoline-3,4'-pyrazolo[3,4-b]pyridine)-5'-carbonitriles as p53-MDM2 interaction inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hany S Ibrahim,Wagdy M Eldehna,Anna Lucia Fallacara,Esam R Ahmed,Hazem A Ghabbour,Mahmoud M Elaasser,Maurizio Botta,Sahar M Abou-Seri,Hatem A Abdel-Aziz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human p21 ELISA Kit

ELISA - Human p21 ELISA Kit (AB214658)

ELISA - Human p21 ELISA Kit (AB214658)

ELISA - Human p21 ELISA Kit (AB214658)

ELISA - Human p21 ELISA Kit (AB214658)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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