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AB313917

Human p27 Kip 1 ELISA Kit

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Human p27 Kip 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human p27 Kip 1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

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Sandwich ELISA - Human p27 Kip 1 ELISA Kit (AB313917)
  • sELISA

Supplier Data

Sandwich ELISA - Human p27 Kip 1 ELISA Kit (AB313917)

Example of standard curve using ab313917. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.03 ng/mL

Range

0.03 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human p27 Kip 1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p27 Kip 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human p27 Kip 1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "103 - 126 %", "average": "= 115" }, { "sample": "Plasma", "range": "99 - 131 %", "average": "= 109.5" }, { "sample": "Cell culture media", "range": "71 - 99 %", "average": "= 83.44" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313917-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313917-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human p27 Kip 1 Protein Standard", "number":"AB313917-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313917-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human p27 Kip 1 Antibody-coated ELISA Plate", "number":"AB313917-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313917-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human p27 Kip 1 Detection Antibody", "number":"AB313917-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313917-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313917-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
Biological function summary

The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.

Pathways

The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.

Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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Product protocols

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Target data

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed : 28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
See full target information CDKN1B
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB32034

Anti-p27 KIP 1 antibody [Y236]

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [Y236] (AB32034)

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Proteins & Peptides

AB113602

Recombinant Human p27 KIP 1 protein

SDS-PAGE - Recombinant Human p27 KIP 1 protein (AB113602)

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