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Human p53 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human p53 in Human in Plasma, Cell Lysate, Serum samples.

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Sandwich ELISA - Human p53 ELISA Kit (AB46067), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell Lysate, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.1 - 100 U/mL
Assay time
3h 45m
Sensitivity
< 1.5 U/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

What's included?

1 x 96 Tests
Components
10X Standard Diluent Buffer
1 x 15 mL
200X Wash Buffer
1 x 10 mL
Biotinylated Antibody Diluent
1 x 7.5 mL
Biotinylated anti p53
1 x 400 µL
Chromogen TMB Substrate Solution
1 x 11 mL
Control
2 x 1 Vial
HRP Diluent
1 x 12 mL
Standard Diluent (Serum)
1 x 7 mL
Stop Reagent
1 x 11 mL
Streptavidin-HRP
2 x 5 µL
p53 Microplate (12 x 8 well strips)
1 x 1 Unit
p53 Standard (Lyophilized)
2 x 1 Vial

Recommended products

Human p53 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human p53 in Human in Plasma, Cell Lysate, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell Lysate, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.1 - 100 U/mL
Assay time
3h 45m
Assay Platform
Microplate
Sensitivity
< 1.5 U/mL

Precision

Intra assay

Sample
Sample A
n
8
mean
53.6 U/mL
SD
1.39
C.V.
= 2.6
Sample
Sample B
n
8
mean
14.7 U/mL
SD
0.62
C.V.
= 4.2

Inter assay

Sample
Sample A
n
5
mean
29.1 U/mL
SD
2
C.V.
= 6.8
Sample
Sample B
n
4
mean
5.87 U/mL
SD
0.55
C.V.
= 9.3

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Abcam's Human p53 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of p53 in serum, plasma and cell lysates.

A monoclonal antibody specific p53 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known p53 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for p53 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of p53 present in the samples.

This kit will recognize both endogenous and recombinant Human p53.

Get results in 90 minutes with Human p53 ELISA Kit (Human p53 ELISA Kit ab171571) from our SimpleStep ELISA® range.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

Associated diseases and disorders

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

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1 product image

  • Sandwich ELISA - Human p53 ELISA Kit (ab46067), expandable thumbnail

    Sandwich ELISA - Human p53 ELISA Kit (ab46067)

    Representative standard curve using ab46067

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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