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AB46067

Human p53 ELISA Kit

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(10 Publications)

Human p53 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human p53 in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

1 Images
Sandwich ELISA - Human p53 ELISA Kit (AB46067)
  • sELISA

Unknown

Sandwich ELISA - Human p53 ELISA Kit (AB46067)

Representative standard curve using ab46067

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell Lysate, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 U/mL

Range

3.1 - 100 U/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human p53 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of p53 in serum, plasma and cell lysates.

A monoclonal antibody specific p53 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known p53 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for p53 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of p53 present in the samples.

This kit will recognize both endogenous and recombinant Human p53.

Get results in 90 minutes with Human p53 ELISA Kit (ab171571) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "53.6 U/mL", "standardDeviation": "1.39", "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 5, "mean": "29.1 U/mL", "standardDeviation": "2", "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 4, "mean": "5.87 U/mL", "standardDeviation": "0.55", "coefficientOfVariability": "= 9.3" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "14.7 U/mL", "standardDeviation": "0.62", "coefficientOfVariability": "= 4.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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Product protocols

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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0303736 PubMed40179064

2025

Elucidating the impact of Hypericum alpestre extract and L-NAME on the PI3K/Akt signaling pathway in A549 lung adenocarcinoma and MDA-MB-231 triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hayarpi Javrushyan,Mikayel Ginovyan,Tigran Harutyunyan,Smbat Gevorgyan,Zaruhi Karabekian,Alina Maloyan,Nikolay Avtandilyan

Environment international 171:107695 PubMed36574746

2022

The association between ambient particulate matter exposure and the telomere-mitochondrial axis of aging in newborns.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Van Der Stukken,Tim S Nawrot,Congrong Wang,Wouter Lefebvre,Charlotte Vanpoucke,Michelle Plusquin,Harry A Roels,Bram G Janssen,Dries S Martens

Biology 11: PubMed36101331

2022

Ferroptosis-Related Proteins Are Potential Diagnostic Molecular Markers for Patients with Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Meiting Shi,Xiaofeng Yang,Yuzhen Ding,Lu Sun,Ping Zhang,Mengyuan Liu,Xiaoxue Han,Zhengrui Huang,Ruiman Li

Aging 14:1627-1650 PubMed35169104

2022

The telomere-mitochondrial axis of aging in newborns.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Van Der Stukken,Tim S Nawrot,Rossella Alfano,Congrong Wang,Sabine A S Langie,Michelle Plusquin,Bram G Janssen,Dries S Martens

Diabetes, metabolic syndrome and obesity : targets and therapy 14:4233-4240 PubMed34675574

2021

LncRNA LINC00673 is Downregulated in Diabetic Retinopathy and Regulates the Apoptosis of Retinal Pigment Epithelial Cells via Negatively Regulating p53.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Cheng,Yanxia Zhu,Linli Ma

Experimental and therapeutic medicine 22:734 PubMed34055051

2021

lncRNA WT1-AS is upregulated in osteoporosis and regulates the apoptosis of osteoblasts by interacting with p53.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoqun Wang,Quan Xie,Wen Sun,Ying Zhou,Yu Liu

Experimental and therapeutic medicine 20:225 PubMed33193839

2020

lncRNA ZEB1-AS1 is downregulated in diabetic lung and regulates lung cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhi Gu,Hong Sun,Zhuan Yan

Annals of agricultural and environmental medicine : AAEM 27:585-590 PubMed33356065

2020

Oxidative toxic stress and p53 level in healthy subjects occupationally exposed to outdoor air Pollution - a cross-sectional study in Iran.

Applications

Unspecified application

Species

Unspecified reactive species

Noushin Rezaei Vandchali,Ali Koolivand,Akram Ranjbar,Parvin Zarei,Mojtaba Fathi,Sheida Malekafzali,Nargess Mollamohammadi,Farideh Jalali-Mashayekhi

Oncology letters 15:8816-8824 PubMed29805621

2018

miR-19 in blood plasma reflects lung cancer occurrence but is not specifically associated with radon exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Bulgakova,Dinara Zhabayeva,Assiya Kussainova,Alessandra Pulliero,Alberto Izzotti,Rakhmetkazhi Bersimbaev

Asian Pacific journal of cancer prevention : APJCP 18:2551-2553 PubMed28952299

2017

No Evaluation of Serum P53 Levels in Iraqi Female Breast Cancer Patients

Applications

Unspecified application

Species

Unspecified reactive species

Ferdous A Jabir,Wisam H Hoidy
View all publications
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