Human p53 ELISA Kit (pSer15) is a Sandwich (quantitative) ELISA kit for the measurement of Human p53 (pSer15) in Human in Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells, Cell Lysate samples.
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- Molecules (Basel, Switzerland) 26:2021Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcela Vlcnovska et. al.PubMed 33530345
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 4h 15m
- Sensitivity
- = 8 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:35618207, PubMed:36634798, PubMed:38653238, PubMed:9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17189187, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:38653238, PubMed:9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:24652652, PubMed:9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Alternative names
P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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Human p53 ELISA Kit (pSer15) is a Sandwich (quantitative) ELISA kit for the measurement of Human p53 (pSer15) in Human in Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 4h 15m
- Assay Platform
- Microplate
- Sensitivity
- = 8 µg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 8.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 14.2 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's p53 pSer15 Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the accurate quantitative measurement of phosphorylated Ser15 of p53 protein in human cell and tissue lysates. The assay employs an antibody specific to p53 protein coated onto well plate strips. Standards and samples are pipetted into the wells and p53 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-p53 phospho Ser15 detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of phosphorylated Ser15 of bound p53. The reaction is stopped by adding hydrochloric acid which changes the color from blue to yellow and the color intensity is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human p53 ELISA Kit (Human p53 ELISA Kit ab171571) from our SimpleStep ELISA® range.
p53 (TP53 gene) acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling cross-over.
The p53 protein is found in increased amounts in a wide variety of transformed cells. p53 is mutated or inactivated in about 60% of cancers. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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4 product images
Sandwich ELISA - Human p53 ELISA Kit (pSer15) (ab156027)
Example positive control sample standard curve. A dilution series of extract in Incubation Buffer in the working range of the assay. The extract was prepared from pellets of Hek293T cells treated with etoposide (Etoposide, Topoisomerase II inhibitor ab120227).
Sandwich ELISA - Human p53 ELISA Kit (pSer15) (ab156027)
Example experimental analysis of drug treatment of Hek 293T and MCF7 cells. Cells were treated with calyculin A + okadaic acid (Cal A + OA), etoposide (Etoposide, Topoisomerase II inhibitor ab120227), camptothecin or drugs’ vehicles, as indicated. Diluted cell extracts were analyzed ab156027 (A, C) and p53 protein ELISA (B, D), using ab117995. Extract of Hek 293T treated with etoposide was used for positive control sample standard curves. Relative levels interpolated from standard curves and expressed in percent of etoposide- (A, B) and camptothecin- (C, D) treated samples are shown.
Sandwich ELISA - Human p53 ELISA Kit (pSer15) (ab156027)
The p53 pSer15 ELISA specifically measures the phosphorylated Serine. Extracts of Hek 293T cells (induced with etoposide) were treated with increasing concentrations of λ protein phosphatase (400x= 400-times diluted, 100x=100-times diluted, 25x=25-times diluted), or left untreated (Contr), and relative phospho Ser15 levels were determined using this kit. Dilutions of extracts of Hek 293T cells treated with etoposide were used to construct the standard curve.
Western blot - Human p53 ELISA Kit (pSer15) (ab156027)
Hek293T cells were treated with vehicle (lane 1) or etoposide (lanes 2-6). MCF7 cells were treated with vehicle (lane 7) or camptothecin for 6 (lane 8), 16 (lane 9) and 24 (lane 10) hours. Extracts of Hek293T cells (induced with etoposide) were treated with increasing concentrations of λ protein phosphatase (lane 4, 400x diluted; lane 5, 100x diluted; lane 6, 25x diluted) or left untreated (lane 3). Samples (lanes 1-2 and 7-10, 20 μg/lane; lanes 3-6, 8 μg/lane) were analyzed by Western blotting using the p53 pSer15 Detector Antibody of ab156027 kit (A), or a p53 antibody (Anti-p53 antibody [DO-1] - ChIP Grade ab1101) was used to detect total p53 protein (B).
The detector antibody used in this kit specifically detects the phosphorylated p53 as determined by Western blotting.
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