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AB287164

Human p90RSK1 ELISA Kit

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Human p90RSK1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human p90RSK1 with a sensitivity of 70.41 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MAPKAPK1A, RSK1, RPS6KA1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1

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Sandwich ELISA - Human p90RSK1 ELISA Kit (AB287164)
  • sELISA

Supplier Data

Sandwich ELISA - Human p90RSK1 ELISA Kit (AB287164)

Interpolated concentrations of human RSK1 p90 in HeLa cell extract and human colon tissue extract.

Interpolated concentration of native RSK1 p90 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 31.25 μg/mL and human colon tissue extract 62.50 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human p90RSK1 ELISA Kit (AB287164)
  • sELISA

Supplier Data

Sandwich ELISA - Human p90RSK1 ELISA Kit (AB287164)

Example of human RSK1 p90 standard curve in 1X Cell Extraction Buffer PTR.

Example of human RSK1 p90 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 70.41 pg/mL

Range

375 - 24000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32114

Anti-RSK1 p90 antibody [E4]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RSK1 p90 antibody [E4] (AB32114)

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Assay Kits

AB279915

Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)

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Proteins & Peptides

AB60880

Recombinant human RSK1 p90 protein

Functional Studies - Recombinant human RSK1 p90 protein (AB60880)

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Primary Antibodies

AB109115

Anti-Huntingtin antibody [EPR5526]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-Huntingtin antibody [EPR5526] (AB109115)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human p90RSK1 ELISA kit (ab287164) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human p90RSK1 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human p90RSK1 with 70.41 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "106 - 110 %", "average": "= 109" }, { "sample": "Cell Lysate", "range": "104 - 107 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287164-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB287164-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287164-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287164-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human RSK1 p90 Lyophilized Recombinant Protein", "number":"AB287164-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RSK1 p90 Capture Antibody", "number":"AB287164-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287164-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB287164-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287164-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RSK1 p90 Detector Antibody", "number":"AB287164-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB287164-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287164-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287164-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSK1 also known as p90 ribosomal S6 kinase 1 or p90RSK is a serine/threonine kinase with an approximate mass of 90 kDa. It belongs to the family of proteins that integrate signals from various extracellular stimuli. This kinase is expressed in diverse tissues including brain lung liver and skeletal muscle. At the molecular level RSK1 phosphorylates various target proteins that are involved in regulating cellular growth and survival. Its activity is regulated by phosphorylation events that occur at specific sites such as Serine 238 (E238 in some literature) which are critical for its function.
Biological function summary

RSK1 plays a fundamental role in cell proliferation and differentiation in the context of the p90 protein weight. It is an important component of the mitogen-activated protein kinase (MAPK) signaling pathway and forms part of multi-protein complexes that facilitate its activation and function. RSK1 influences various biological processes by phosphorylating transcription factors histone proteins and other kinases thereby regulating gene expression and protein synthesis. These actions highlight its influence in maintaining cellular homeostasis.

Pathways

RSK1 p90 interacts significantly with the MAPK/ERK and PI3K/AKT pathways. It acts downstream of ERK where it receives activating signals that enable it to phosphorylate substrates within the cell. This role supports its interaction with proteins like Akt and mTOR which are critical mediators of cell growth signals. The interplay between these pathways enables cells to respond to growth factors and stress contributing to the regulation of development and metabolism.

RSK1 is implicated in the progression of cancer and neurological disorders. In cancer abnormal RSK1 signaling can lead to unchecked cell growth and survival where it often interfaces with proteins such as C-RAF and BCL-2 to promote oncogenic pathways. In neurological disorders RSK1 influences pathways that regulate neuron survival and plasticity connecting it to proteins like CREB. Understanding RSK1's role in these conditions can provide insight into targeted therapies that may mitigate these diseases.
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Product protocols

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Target data

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed : 10679322, PubMed : 12213813, PubMed : 15117958, PubMed : 16223362, PubMed : 17360704, PubMed : 18722121, PubMed : 26158630, PubMed : 35772404, PubMed : 9430688). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes (PubMed : 18508509, PubMed : 18813292). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed : 12213813, PubMed : 16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed : 18508509, PubMed : 18813292). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex (PubMed : 17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed : 16763566). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed : 15342917). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed : 22017876). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed : 10679322, PubMed : 16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed : 11684016). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed : 18508509, PubMed : 18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (PubMed : 18508509, PubMed : 18813292). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed : 26158630). In response to mTORC1 activation, phosphorylates EIF4B at 'Ser-406' and 'Ser-422' which stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function (PubMed : 35772404).. (Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated.
See full target information RPS6KA1
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websiteProtocolBooklet
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