Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Human PACAP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PACAP in Human in Serum, Plasma, Cell culture supernatant samples.
Colorimetric
Serum, Plasma, Cell culture supernatant
Sandwich (quantitative)
Human
3.65 - 1000 pg/mL
= 3.65 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.
Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1, MZB1, MEDA7, PACAP, HSPC190
Human PACAP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PACAP in Human in Serum, Plasma, Cell culture supernatant samples.
Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1, MZB1, MEDA7, PACAP, HSPC190
Colorimetric
Serum, Plasma, Cell culture supernatant
Sandwich (quantitative)
Human
3.65 - 1000 pg/mL
Pre-coated microplate (12 x 8 well strips)
= 3.65 pg/mL
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 121.7 | Range 113 - 130 % |
Sample type Plasma | Average % = 119.2 | Range 110 - 129 % |
Sample type Cell culture supernatant | Average % = 123.2 | Range 115 - 135 % |
Sample type Cell culture media | Average % = 136.6 | Range 130 - 139 % |
Blue Ice
-20°C
-20°C
Human PACAP ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PACAP in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human PACAP coated on a 96-well plate.
PACAP exerts multiple effects by binding to three G protein-coupled receptors: PAC1 VPAC1 and VPAC2. It influences processes like neuroprotection neurodevelopment and neurotransmission. As part of the PACAP/VIP receptor complex it regulates cyclic AMP production and calcium signaling mediating its effects in both neural and non-neural tissues. It is particularly involved in paired functions with neurotransmitters and other signaling molecules indicating its key role in maintaining homeostasis.
PACAP also known as Pituitary Adenylate Cyclase-Activating Polypeptide is a neuropeptide functioning as a neurotransmitter and neuromodulator. It belongs to the vasoactive intestinal peptide (VIP)/secretin/glucagon hormone family. PACAP consists of 38 amino acid residues and has a molecular mass of approximately 4.5 kDa. It is expressed in various regions including the central nervous system (CNS) endocrine cells and peripheral tissues. This broad expression pattern supports its diverse role in physiological and neurobiological processes.
The PACAP signaling mechanism integrates into the cAMP-dependent pathway and the MAPK/ERK pathway. These pathways are central to cell proliferation differentiation and survival. PACAP's interaction with MAPK/ERK pathway involves proteins such as Raf and MEK further modulating gene expression and synaptic plasticity. By this mechanism PACAP plays a vital role in modulating neurotransmission and neuroendocrine functions.
PACAP is linked to migraine and neurodegenerative diseases like Alzheimer's disease. Alterations in PACAP signaling can affect pain pathways and neuroinflammation potentially exacerbating such conditions. PACAP's interaction with proteins like CGRP (Calcitonin Gene-Related Peptide) is significant as both are implicated in the pathophysiology of migraines. Moreover in Alzheimer’s disease the dysregulation of PACAP and its receptors contributes to neuroinflammation and neurodegeneration.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of standard curve using ab309452. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com