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Human PACAP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PACAP in Human in Serum, Plasma, Cell culture supernatant samples.

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Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

3.65 - 1000 pg/mL

Sensitivity

= 3.65 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.

Alternative names

What's included?

1 x 96 Tests
Components
100X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
96-Well Plate Sealing Film
1 x 1 Unit
Biotinylated Human PACAP Detection Antibody
2 x 1 Vial
Human PACAP Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human PACAP Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human PACAP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PACAP in Human in Serum, Plasma, Cell culture supernatant samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

3.65 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3.65 pg/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 121.7

Range

113 - 130 %

Sample type

Plasma

Average %

= 119.2

Range

110 - 129 %

Sample type

Cell culture supernatant

Average %

= 123.2

Range

115 - 135 %

Sample type

Cell culture media

Average %

= 136.6

Range

130 - 139 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human PACAP ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PACAP in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human PACAP coated on a 96-well plate.

Supplementary info

Biological function summary

PACAP exerts multiple effects by binding to three G protein-coupled receptors: PAC1 VPAC1 and VPAC2. It influences processes like neuroprotection neurodevelopment and neurotransmission. As part of the PACAP/VIP receptor complex it regulates cyclic AMP production and calcium signaling mediating its effects in both neural and non-neural tissues. It is particularly involved in paired functions with neurotransmitters and other signaling molecules indicating its key role in maintaining homeostasis.

Activity summary

PACAP also known as Pituitary Adenylate Cyclase-Activating Polypeptide is a neuropeptide functioning as a neurotransmitter and neuromodulator. It belongs to the vasoactive intestinal peptide (VIP)/secretin/glucagon hormone family. PACAP consists of 38 amino acid residues and has a molecular mass of approximately 4.5 kDa. It is expressed in various regions including the central nervous system (CNS) endocrine cells and peripheral tissues. This broad expression pattern supports its diverse role in physiological and neurobiological processes.

Pathways

The PACAP signaling mechanism integrates into the cAMP-dependent pathway and the MAPK/ERK pathway. These pathways are central to cell proliferation differentiation and survival. PACAP's interaction with MAPK/ERK pathway involves proteins such as Raf and MEK further modulating gene expression and synaptic plasticity. By this mechanism PACAP plays a vital role in modulating neurotransmission and neuroendocrine functions.

Associated diseases and disorders

PACAP is linked to migraine and neurodegenerative diseases like Alzheimer's disease. Alterations in PACAP signaling can affect pain pathways and neuroinflammation potentially exacerbating such conditions. PACAP's interaction with proteins like CGRP (Calcitonin Gene-Related Peptide) is significant as both are implicated in the pathophysiology of migraines. Moreover in Alzheimer’s disease the dysregulation of PACAP and its receptors contributes to neuroinflammation and neurodegeneration.

Product promise

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1 product image

  • Sandwich ELISA - Human PACAP ELISA Kit (ab309452), expandable thumbnail

    Sandwich ELISA - Human PACAP ELISA Kit (ab309452)

    Example of standard curve using ab309452. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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