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AB275904

Human PAEP ELISA Kit (Glycodelin)

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Human PAEP ELISA Kit (Glycodelin) is a single-wash 90-min Simplestep used to quantify Human PAEP (Glycodelin) with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glycodelin, GD, Placental protein 14, Pregnancy-associated endometrial alpha-2 globulin, Progestagen-associated endometrial protein, Progesterone-associated endometrial protein, Zona-binding inhibitory factor-1, PP14, PAEG, PEG, ZIF-1, PAEP

2 Images
Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)

Example of Human PAEP standard curve in Sample Diluent NS

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)

Interpolated concentrations of native PAEP in Human serum samples

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human PAEP ELISA kit (ab275904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAEP protein in human serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAEP with 3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PAEP, progestogen-associated endometrial protein also known as glycodelin, is a glycoprotein which plays many important roles in fertility and maintenance of pregnancy. PAEP changes its biochemical interaction based on its glycosylation state. PAEP is a prognostic marker for in vitro fertilization and for premature membrane rupture. The antibodies in this kit were raised against a recombinant full-length mature protein. This protein does not have a homolog in mouse or rat and is not expected to cross-react with samples from these species.

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.98" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

Recovery

[ { "sample": "Serum", "range": "89 - 116 %", "average": "= 107" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275904-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275904-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275904-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275904-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275904-CMP09", "productcode":"" }, { "size":"1 x 600 mL", "name":"10X Human PAEP (Glycodelin) Capture Antibody", "number":"AB275904-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB275904-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAEP (Glycodelin) Lyophilized Recombinant Protein", "number":"AB275904-CMP08", "productcode":"" }, { "size":"1 x 600 mL", "name":"10X Human PAEP (Glycodelin) Detector Antibody", "number":"AB275904-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275904-CMP05", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Glycoprotein that regulates critical steps during fertilization and also has immunomonomodulatory effects. Four glycoforms, namely glycodelin-S, -A, -F and -C have been identified in reproductive tissues that differ in glycosylation and biological activity. Glycodelin-A has contraceptive and immunosuppressive activities (PubMed : 7531163, PubMed : 9918684). Glycodelin-C stimulates binding of spermatozoa to the zona pellucida (PubMed : 17192260). Glycodelin-F inhibits spermatozoa-zona pellucida binding and significantly suppresses progesterone-induced acrosome reaction of spermatozoa (PubMed : 12672671). Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa (PubMed : 15883155).
See full target information Glycodelin
websiteProtocolBooklet
en

Product promise

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