Human PAI1 ELISA Kit

Abcam's PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in Human plasma.

PAI1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.

Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate or be detected. Unbound PAI1 samples are aspirated and an anti-PAI1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.

Get results in 90 minutes with Human PAI1 ELISA Kit (SERPINE1) (ab184863) from our SimpleStep ELISA^® range.