AB108891

Human PAI1 ELISA Kit (SERPINE1)

Name: Human PAI1 ELISA Kit (SERPINE1) (ab108891) | Abcam
Brand: Abcam Limited
SKU: AB108891

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(10 Publications)

Human PAI1 ELISA Kit (SERPINE1) is a Sandwich ELISA for the measurement of Human PAI1 (SERPINE1) in Human in Biofluids samples.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

4 Images

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

representative standard curve of ab108891

sELISA

Lab

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.

Standard curve mean of duplicates (+/- SD) with background readings subtracted

sELISA

Lab

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.

PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested

sELISA

Lab

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.

PAI1 measured in various samples showing quantity (ng) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14 pg/mL

Range

0.039 - 2.5 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum and CSF.

A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.

This assay recognizes both natural and recombinant Human PAI-1.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108891-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108891-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108891-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PAI1 Standard", "number":"AB108891-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PAI1 Microplate (12 x 8 well strips)", "number":"AB108891-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108891-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108891-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human PAI1 Antibody", "number":"AB108891-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108891-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108891-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108891-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

See full target information SERPINE1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

mBio 13:e0089222 PubMed35532162

2022

Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Ebba Rosendal,Ionut Sebastian Mihai,Miriam Becker,Debojyoti Das,Lars Frängsmyr,B David Persson,Gregory D Rankin,Remigius Gröning,Johan Trygg,Mattias Forsell,Johan Ankarklev,Anders Blomberg,Johan Henriksson,Anna K Överby,Annasara Lenman

Journal of thrombosis and haemostasis : JTH 20:1008-1014 PubMed35102689

2022

Non-severe COVID-19 is associated with endothelial damage and hypercoagulability despite pharmacological thromboprophylaxis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Kelliher,Luisa Weiss,Sarah Cullivan,Ellen O'Rourke,Claire A Murphy,Shane Toolan,Áine Lennon,Paulina B Szklanna,Shane P Comer,Hayley Macleod,Ana Le Chevillier,Sean Gaine,Kate M A O'Reilly,Brian McCullagh,John Stack,Patricia B Maguire,Fionnuala Ní Áinle,Barry Kevane

Acta neuropathologica communications 8:35 PubMed32192527

2020

Altered secretory and neuroprotective function of the choroid plexus in progressive multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabela Rodríguez-Lorenzo,David Miguel Ferreira Francisco,Ricardo Vos,Bert van Het Hof,Merel Rijnsburger,Horst Schroten,Hiroshi Ishikawa,Wissam Beaino,Rémy Bruggmann,Gijs Kooij,Helga E de Vries

Developmental cell 47:409-424.e9 PubMed30458137

2018

Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Arnandis,Pedro Monteiro,Sophie D Adams,Victoria Louise Bridgeman,Vinothini Rajeeve,Emanuela Gadaleta,Jacek Marzec,Claude Chelala,Ilaria Malanchi,Pedro R Cutillas,Susana A Godinho

Experimental and therapeutic medicine 15:5125-5132 PubMed29805539

2018

MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Jie Hu,Xingpeng Chen

Journal of natural products 80:2969-2986 PubMed29087712

2017

Grassystatins D-F, Potent Aspartic Protease Inhibitors from Marine Cyanobacteria as Potential Antimetastatic Agents Targeting Invasive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma H Al-Awadhi,Brian K Law,Valerie J Paul,Hendrik Luesch

Diabetes/metabolism research and reviews 33: PubMed28806491

2017

The association between dysregulated adipocytokines in early pregnancy and development of gestational diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Sally K Abell,Soulmaz Shorakae,Cheryce L Harrison,Danielle Hiam,Alba Moreno-Asso,Nigel K Stepto,Barbora De Courten,Helena J Teede

Experimental and therapeutic medicine 11:1993-1998 PubMed27168840

2016

Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuqin Li,Yong Gao,Zhaoyun Meng,Cui Zhang,Qinde Qi

Nutrients 6:3187-201 PubMed25111123

2014

Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD.

Applications

Unspecified application

Species

Human

Ran Jin,Jean A Welsh,Ngoc-Anh Le,Jeffrey Holzberg,Puneet Sharma,Diego R Martin,Miriam B Vos

Molecular cancer research : MCR 12:322-34 PubMed24464915

2014

PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Evan Gomes Giacoia,Makito Miyake,Adrienne Lawton,Steve Goodison,Charles J Rosser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PAI1 ELISA Kit (SERPINE1)

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB108891)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

Product promise