Human PAI1 ELISA Kit (SERPINE1) is a Sandwich (quantitative) ELISA kit for the measurement of Human PAI1 (SERPINE1) in Human in Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue samples.
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- mBio 13:e00892222022Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEbba Rosendal,Ionut Sebastian Mihai,Miriam Becker,Debojyoti Das,Lars Frängsmyr,B David Persson,Gregory D Rankin,Remigius Gröning,Johan Trygg,Mattias Forsell,Johan Ankarklev,Anders Blomberg,Johan Henriksson,Anna K Överby,Annasara LenmanPubMed 35532162
- Journal of thrombosis and haemostasis : JTH 20:1008-10142022Non-severe COVID-19 is associated with endothelial damage and hypercoagulability despite pharmacological thromboprophylaxis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Kelliher,Luisa Weiss,Sarah Cullivan,Ellen O'Rourke,Claire A Murphy,Shane Toolan,Áine Lennon,Paulina B Szklanna,Shane P Comer,Hayley Macleod,Ana Le Chevillier,Sean Gaine,Kate M A O'Reilly,Brian McCullagh,John Stack,Patricia B Maguire,Fionnuala Ní Áinle,Barry KevanePubMed 35102689
- Acta neuropathologica communications 8:352020Altered secretory and neuroprotective function of the choroid plexus in progressive multiple sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabela Rodríguez-Lorenzo et. al.PubMed 32192527
- Developmental cell 47:409-424.e92018Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Arnandis,Pedro Monteiro,Sophie D Adams,Victoria Louise Bridgeman,Vinothini Rajeeve,Emanuela Gadaleta,Jacek Marzec,Claude Chelala,Ilaria Malanchi,Pedro R Cutillas,Susana A GodinhoPubMed 30458137
- Experimental and therapeutic medicine 15:5125-51322018MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Li et. al.PubMed 29805539
- Journal of natural products 80:2969-29862017Grassystatins D-F, Potent Aspartic Protease Inhibitors from Marine Cyanobacteria as Potential Antimetastatic Agents Targeting Invasive Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatma H Al-Awadhi et. al.PubMed 29087712
- Diabetes/metabolism research and reviews 33:2017The association between dysregulated adipocytokines in early pregnancy and development of gestational diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSally K Abell,Soulmaz Shorakae,Cheryce L Harrison,Danielle Hiam,Alba Moreno-Asso,Nigel K Stepto,Barbora De Courten,Helena J TeedePubMed 28806491
- Experimental and therapeutic medicine 11:1993-19982016Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuqin Li et. al.PubMed 27168840
- Nutrients 6:3187-2012014Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD.
- Molecular cancer research : MCR 12:322-342014PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvan Gomes Giacoia et. al.PubMed 24464915
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.15 - 1.25 ng/mL
- Assay time
- 4h
- Sensitivity
- = 19 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
What's included?
Recommended products
Human PAI1 ELISA Kit (SERPINE1) is a Sandwich (quantitative) ELISA kit for the measurement of Human PAI1 (SERPINE1) in Human in Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.15 - 1.25 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 19 pg/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 3.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 8.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.
This assay recognizes both natural and recombinant Human PAI-1.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (ab108891)
Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.
Standard curve mean of duplicates (+/- SD) with background readings subtracted
Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (ab108891)
Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.
PAI1 measured in various samples showing quantity (ng) per mL of tested sample
Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (ab108891)
Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit.
PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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