Human PAI1 ELISA Kit (SERPINE1)

• ELISA

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ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Linearity of dilution.

Native PAI1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Interpolated concentrations of native PAI1 in human serum and plasma samples.

The concentrations of PAI1 were measured in duplicates, interpolated from the PAI1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 32.4 ng/mL in serum, 36.6 ng/mL in plasma (citrate), 11.1 ng/mL in plasma (EDTA), and 10.1 ng/mL in plasma (heparin).

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native PAI1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Example of human PAI1 standard curve in Sample Diluent NS.

The PAI1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Interpolated concentrations of native PAI1 in HUVEC cell culture supernatant, and HepG2 cell culture supernatant samples.

The concentrations of PAI1 were measured in duplicates, interpolated from the PAI1 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC cell culture supernatant 1 : 2,000 and HepG2 cell culture supernatant 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 2,061 ng/mL in HUVEC cell culture supernatant and 739 ng/mL in HepG2 cell culture supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Interpolated concentrations of native PAI1 in human stimulated PBMC cell culture supernatant and unstimulated PBMC cell culture supernatant samples.

The concentrations of PAI1 were measured in duplicates, interpolated from the PAI1 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC cell culture supernatant 50% and unstimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 1,896 pg/mL in stimulated PBMC cell culture supernatant and 642 pg/mL in unstimulated PBMC cell culture supernatant.

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Human PAI1 standard curve comparison.

Standard Curve comparison between human PAI1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• sELISA

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Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 24 ng/mL with a range of 8 – 59 ng/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Example of human PAI1 standard curve in 1X Cell Extraction Buffer PTR.

The PAI1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Linearity of dilution.

Native PAI1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Linearity of dilution.

Native PAI1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Example of human PAI1 standard curve in Sample Diluent NS.

The PAI1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Interpolated concentrations of native PAI1 in HUVEC cell extract and HepG2 cell extract samples based on a 500 μg/mL and 50 μg/mL extract load, respectively.

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 677 pg/mL in HUVEC cell extract and 989 pg/mL in HepG2 cell extract.

• sELISA

Supplier Data

Sandwich ELISA - Human PAI1 ELISA Kit (SERPINE1) (AB269373)

Example of human PAI1 standard curve in 1X Cell Extraction Buffer PTR.

The PAI1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.