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AB108892

Human PAI1 + tPA ELISA Kit

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(2 Publications)

Human PAI1 + tPA ELISA Kit is a Sandwich ELISA for the measurement of Human PAI1 + tPA in Human in Biofluids samples.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT

1 Images
Sandwich ELISA - Human PAI1 + tPA ELISA Kit (AB108892)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAI1 + tPA ELISA Kit (AB108892)

Representative Standard Curve using ab108892.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 34 pg/mL

Range

0.063 - 4 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma and serum samples.

A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.

Get results in 90 minutes with Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108892-1x96Tests", "assetComponentDetails": [ { "size":"1 x 75 µL", "name":"80X Biotinylated Human PAI1 + tPA Antibody", "number":"AB108892-CMP02", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108892-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PAI1 + tPA Standard", "number":"AB108892-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108892-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108892-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108892-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PAI1 + tPA Microplate (12 x 8 well strips)", "number":"AB108892-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB108892-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108892-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).
See full target information SERPINE1

Additional targets

PLAT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets 12:485-493 PubMed31043798

2019

Circulating microparticle subpopulation in metabolic syndrome: relation to oxidative stress and coagulation markers.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa M Zahran,Sohair K Sayed,Heba A Abd El Hafeez,Walaa A Khalifa,Nahed A Mohamed,Helal F Hetta

The Journal of infectious diseases 208:218-23 PubMed23570848

2013

tPA Alu (I/D) polymorphism associates with bacterial osteomyelitis.

Applications

Unspecified application

Species

Human

Eulalia Valle-Garay,Angel H Montes,Jose R Corte,Alvaro Meana,Joshua Fierer,Victor Asensi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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