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AB269559

Human PAI1+tPA ELISA Kit

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Human PAI1+tPA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PAI1+tPA with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

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Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 + tPA concentration was determined to be 7 ng/mL with a range of 3 – 12 ng/mL.

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)

Human PAI1+tPA standard curve comparison.

Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab92151) and current human MMP3 SimpleStep ELISA kit (ab269559).

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)

Example of human PAI1 + tPA standard curve in Sample Diluent NS.

The PAI1 + tPA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAI1+tPA ELISA Kit (AB269559)

Interpolated concentrations of native PAI1 + tPA in human serum and plasma samples.

The concentrations of PAI1 + tPA were measured in duplicates, interpolated from the PAI1 + tPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 + tPA concentration was determined to be 6.0 ng/mL in serum, 10.7 ng/mL in plasma (citrate), 4.3 ng/mL in plasma (EDTA) and 5.2 ng/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 18 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human PAI1+tPA ELISA Kit (ab269559) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1+tPA protein in edta plasma, hep plasma, serum, urine, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAI1+tPA with 18 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tissue plasminogen activator (TPA or PLAT) is a serine protease that catalyzes the conversion of plasminogen to plasmin, which is integral to blood clot breakdown. Plasminogen activator inhibitor-1 (PAI1 or SERPINE1) is a serine protease inhibitor that blocks the function of TPA. PAI1 and TPA form a complex that is stable and non-covalent. The PAI1/TPA complex appears to be predictive of risk for myocardial infarction. This product uses a tPA specific capture antibody and a PAI1 specific detector antibody to specifically detect PAI1/TPA complexes in human biological samples. The reference protein is a non-covalent complex of PAI1 and TPA.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "93 - 106 %", "average": "= 98" }, { "sample": "Urine", "range": "97 - 106 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "82 - 87 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "96 - 106 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).
See full target information SERPINE1
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websiteProtocolBooklet
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Product promise

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