Human Pancreatic Polypeptide ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pancreatic Polypeptide in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.
Alternative names
PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y
What's included?
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Human Pancreatic Polypeptide ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pancreatic Polypeptide in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 119 | Range 115 - 123 % |
Sample type Serum | Average % = 107 | Range 103 - 112 % |
Sample type EDTA Plasma | Average % = 82 | Range 81 - 84 % |
Sample type Heparin Plasma | Average % = 93 | Range 87 - 98 % |
Sample type Citrate plasma | Average % = 82 | Range 80 - 84 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Pancreatic Polypeptide ELISA Kit (ab288177) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Pancreatic Polypeptide protein in plasma, serum and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pancreatic Polypeptide with 3.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.
- Biological function summary
In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.
- Pathways
Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.
- Associated diseases and disorders
Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.
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3 product images
Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (ab288177)
Interpolated concentrations of Pancreatic Polypeptide in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Pancreatic Polypeptide concentration was determined to be 349 pg/mL with a range of 17 - 1,248 pg/mL.
Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (ab288177)
Interpolated concentrations of human Pancreatic Polypeptide in serum, plasma (citrate), plasma (heparin) and plasma (EDTA). Interpolated concentration of native Pancreatic Polypeptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 100% plasma (citrate) 100%, plasma (heparin)100%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.
Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (ab288177)
Example of human Pancreatic Polypeptide standard curve in Sample Diluent 25BS. Example of human Pancreatic Polypeptide standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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