AB288177

Human Pancreatic Polypeptide ELISA Kit

Recombinant
SimpleStep
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Human Pancreatic Polypeptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Pancreatic Polypeptide with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y

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Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

sELISA

Supplier Data

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Interpolated concentrations of human Pancreatic Polypeptide in serum, plasma (citrate), plasma (heparin) and plasma (EDTA). Interpolated concentration of native Pancreatic Polypeptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 100% plasma (citrate) 100%, plasma (heparin)100%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

sELISA

Supplier Data

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Interpolated concentrations of Pancreatic Polypeptide in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Pancreatic Polypeptide concentration was determined to be 349 pg/mL with a range of 17 - 1,248 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Example of human Pancreatic Polypeptide standard curve in Sample Diluent 25BS. Example of human Pancreatic Polypeptide standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.7 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Pancreatic Polypeptide ELISA Kit (ab288177) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Pancreatic Polypeptide protein in plasma, serum and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pancreatic Polypeptide with 3.7 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "115 - 123 %", "average": "= 119" }, { "sample": "Serum", "range": "103 - 112 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Heparin Plasma", "range": "87 - 98 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "80 - 84 %", "average": "= 82" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.

Biological function summary

In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.

Pathways

Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.

Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.

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Product protocols

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Target data

Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.

See full target information PPY

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Pancreatic Polypeptide ELISA Kit

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Sandwich ELISA - Human Pancreatic Polypeptide ELISA Kit (AB288177)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise