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AB326751

Human PAP (ACPP) ELISA Kit, Chemiluminescent

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Human PAP (ACPP) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human PAP (ACPP) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.83 - 4000 pg/ml with a sensitivity of 0.65 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

ACPP, ACP3, Prostatic acid phosphatase, PAP, 5'-nucleotidase, Acid phosphatase 3, Ecto-5'-nucleotidase, Protein tyrosine phosphatase ACP3, Thiamine monophosphatase, 5'-NT, TMPase

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Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 416 pg/mL with a range of 94 – 718 pg/mL.

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)

Interpolated concentrations of native PAP in human urine and LNCap cell culture supernatant samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 10%, and LNCap cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 20,039 pg/mL in neat urine and 5,676 pg/mL in neat LNCap cell culture supernatant.

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)

Interpolated concentrations of native PAP in human serum and plasma samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 406 pg/mL in neat serum, 331 pg/mL in neat plasma (citrate), 282 pg/mL in neat plasma (EDTA) and 417 pg/mL in neat plasma (heparin).

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)
  • sELISA

Lab

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)

Dynamic range comparison between the Chemiluminescent Human PAP SimpleStep ELISA, colorimetric Human PAP SimpleStep ELISA (ab267802), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)
  • sELISA

Lab

Sandwich ELISA - Human PAP (ACPP) ELISA Kit, Chemiluminescent (AB326751)

Standard curve comparison between the Chemiluminescent Human PAP SimpleStep ELISA and colorimetric Human PAP SimpleStep ELISA (ab267802) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Urine, Cell culture media, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.65 pg/mL

Range

1.83 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human PAP (ACPP) ELISA Kit, Chemiluminescent (ab326751) is a rapid single-wash sandwich 55-min ELISA to measure Human PAP (ACPP) in Cell culture supernatant, Serum, EDTA Plasma, Urine, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 0.65 pg/mL.

How the assay works

Human PAP (ACPP) ELISA Kit, Chemiluminescent (ab326751) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PAP (ACPP) (ab326751) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PAP antibody [EPR22614-64] - BSA and Azide free (Capture) ab269386
- Anti-PAP antibody [EPR22614-226] - BSA and Azide free (Detector) ab269387
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 86 %", "average": "= 86" }, { "sample": "Serum", "range": "84 - 93 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "81 - 98 %", "average": "= 98" }, { "sample": "Urine", "range": "81 - 95 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "89 - 111 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "87 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "80 - 99 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed : 10506173, PubMed : 15280042, PubMed : 20498373, PubMed : 9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed : 10506173, PubMed : 15280042).. Isoform 2. Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed : 20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).. PAPf39. (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed : 18083097, PubMed : 19451623, PubMed : 19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed : 19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed : 19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed : 19403677).
See full target information ACP3
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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