AB267802

Human PAP ELISA Kit (ACPP)

Recombinant
SimpleStep
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(1 Publication)

Human PAP ELISA Kit (ACPP) is a single-wash 90-min Simplestep used to quantify Human PAP (ACPP) with a sensitivity of 9.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ACPP, ACP3, Prostatic acid phosphatase, PAP, 5'-nucleotidase, Acid phosphatase 3, Ecto-5'-nucleotidase, Protein tyrosine phosphatase ACP3, Thiamine monophosphatase, 5'-NT, TMPase

4 Images

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 416 pg/mL with a range of 94 – 718 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Interpolated concentrations of native PAP in human serum and plasma samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 406 pg/mL in neat serum, 331 pg/mL in neat plasma (citrate), 282 pg/mL in neat plasma (EDTA) and 417 pg/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Example of human PAP standard curve in Sample Diluent NS.

The PAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Interpolated concentrations of native PAP in human urine and LNCap cell culture supernatant samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 10%, and LNCap cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 20,039 pg/mL in neat urine and 5,676 pg/mL in neat LNCap cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.73 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PAP ELISA Kit (ACPP) (ab267802) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAP (ACPP) protein in cell culture supernatant, edta plasma, hep plasma, serum, urine, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAP (ACPP) with 9.73 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PAP (Prostatic Acid Phosphatase, EC:3.1.3.2), also known as ACPP is a non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. PAP expression is highest in prostate. PAP is biomarker of prostate cancer as its expression is increased in prostate cancer patients. The isoform 1 is secreted, the isoform 2 is a type I integral membrane protein of plasma membrane and lysosomes. The Isoform 2 function as tumor suppressor through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. The antibodies of this kit are designed to detect both isoforms.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 86 %", "average": "= 99" }, { "sample": "Serum", "range": "84 - 93 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "81 - 98 %", "average": "= 89" }, { "sample": "Urine", "range": "81 - 95 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "89 - 111 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "87 - 106 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "80 - 99 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed : 10506173, PubMed : 15280042, PubMed : 20498373, PubMed : 9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed : 10506173, PubMed : 15280042).. Isoform 2. Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed : 20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).. PAPf39. (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed : 18083097, PubMed : 19451623, PubMed : 19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed : 19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed : 19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed : 19403677).

See full target information ACP3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 12:830691 PubMed35185790

2022

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Xiaoyang Fei,Hongyan Zhang,Wenjing Zhou,Zhaojun Cheng,Ying Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PAP ELISA Kit (ACPP)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Proteins & Peptides

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise